Adeno-Associated Virus Type 2 Rep68 Can Bind to Consensus Rep-Binding Sites on the Herpes Simplex Virus 1 Genome

J Virol. 2015 Nov;89(21):11150-8. doi: 10.1128/JVI.01370-15. Epub 2015 Aug 19.


Adeno-associated virus type 2 is known to inhibit replication of herpes simplex virus 1 (HSV-1). This activity has been linked to the helicase- and DNA-binding domains of the Rep68/Rep78 proteins. Here, we show that Rep68 can bind to consensus Rep-binding sites on the HSV-1 genome and that the Rep helicase activity can inhibit replication of any DNA if binding is facilitated. Therefore, we hypothesize that inhibition of HSV-1 replication involves direct binding of Rep68/Rep78 to the HSV-1 genome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites / genetics
  • Blotting, Western
  • DNA Helicases / metabolism*
  • DNA-Binding Proteins / metabolism*
  • Dependovirus / genetics*
  • Dependovirus / metabolism
  • Genome, Viral / genetics*
  • Herpesvirus 1, Human / genetics*
  • Herpesvirus 1, Human / metabolism
  • Humans
  • Viral Proteins / metabolism*


  • DNA-Binding Proteins
  • Viral Proteins
  • rep proteins, Adeno-associated virus 2
  • DNA Helicases