Complete Genome Sequence of the Novel Temperate Clostridium difficile Phage phiCDIF1296T

Johannes Wittmann; Thomas Riedel; Boyke Bunk; Cathrin Spröer; Sabine Gronow; Jörg Overmann

doi:10.1128/genomeA.00839-15

Complete Genome Sequence of the Novel Temperate Clostridium difficile Phage phiCDIF1296T

Genome Announc. 2015 Aug 20;3(4):e00839-15. doi: 10.1128/genomeA.00839-15.

Authors

Johannes Wittmann¹, Thomas Riedel², Boyke Bunk³, Cathrin Spröer¹, Sabine Gronow³, Jörg Overmann³

Affiliations

¹ Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.
² Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany thomas.riedel@dsmz.de.
³ Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany German Centre of Infection Research (DZIF), partner site Hannover-Braunschweig, Braunschweig, Germany.

Abstract

Clostridium difficile contains many integrated and extrachromosomal genetic elements. In this study, we determined, annotated, and analyzed the complete genome of the C. difficile bacteriophage phiCDIF1296T using single-molecule real-time sequencing technology. To our knowledge, this represents the largest genome (131 kb) of a temperate C. difficile phage recognized so far.