Hemophilus Influenzae Infections with Abscess Formation in Adults: a Retrospective Study Covering an Eleven-Year Period

Clin Lab. 2015;61(7):845-9. doi: 10.7754/clin.lab.2015.141011.

Abstract

Background: The aim of this study was to investigate the presence of Haemophilus influenzae in abscesses in adults and to determine their antibiotic resistance patterns.

Methods: H. influenzae strains isolated from abscesses during an eleven-year period were determined retrospectively and the stored strains were tested for ampicillin, amoxicillin/calvulanic acid, cefuroxime, cefotaxime, ceftriaxone, ciprofloxacin, azithromycin, tetracycline, and imipenem resistance by broth microdilution method. The production of β-lactamase was detected using the nitrocefin disc test and real-time PCR.

Results: A total of 26 H. influenzae isolated strains were found. According to ampicillin resistance and β-lactamase production, 2 strains were determined as BLPAR, 1 strain BLNAR, 1 strain BLPACR, and 22 strains as BLNAS. Cefuroxime resistance was detected in 4 strains, tetracycline resistance was detected in 4 strains, and no resistance to cefotaxime, ceftriaxone, imipenem, azithromycin and levofloxacin was detected.

Conclusions: H. influenzae should be taken into account for the proper management of abscesses.

MeSH terms

  • Abscess / diagnosis
  • Abscess / drug therapy
  • Abscess / microbiology*
  • Anti-Bacterial Agents / therapeutic use
  • DNA, Bacterial / genetics
  • Disk Diffusion Antimicrobial Tests
  • Drug Resistance, Multiple, Bacterial / genetics
  • Genotype
  • Haemophilus Infections / diagnosis
  • Haemophilus Infections / drug therapy
  • Haemophilus Infections / microbiology*
  • Haemophilus influenzae / drug effects
  • Haemophilus influenzae / enzymology
  • Haemophilus influenzae / genetics
  • Haemophilus influenzae / isolation & purification*
  • Humans
  • Phenotype
  • Real-Time Polymerase Chain Reaction
  • Retrospective Studies
  • beta-Lactam Resistance / genetics
  • beta-Lactamases / genetics

Substances

  • Anti-Bacterial Agents
  • DNA, Bacterial
  • beta-Lactamases