Studying the relationship between cell cycle and Alzheimer's disease by gold nanoparticle probes

Chengke Wang; Zhenxin Wang

doi:10.1016/j.ab.2015.08.014

Studying the relationship between cell cycle and Alzheimer's disease by gold nanoparticle probes

Anal Biochem. 2015 Nov 15:489:32-7. doi: 10.1016/j.ab.2015.08.014. Epub 2015 Aug 20.

Authors

Chengke Wang¹, Zhenxin Wang²

Affiliations

¹ School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
² State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China. Electronic address: wangzx@ciac.ac.cn.

PMID: 26299647
DOI: 10.1016/j.ab.2015.08.014

Abstract

In this study, a simple gold nanoparticle (GNP)-based colorimetric assay has been developed for studying the relationship between cell cycle and β-amyloid peptide (Aβ, the biomarker of Alzheimer's disease [AD]) expression level. It was found that Aβ expression of neuronal cells (e.g., SHG-44 cell line) is strongly dependent on cell cycle phases; that is, the Aβ expression level was highest when cells were arrested in the G1/S phase by thymidine and was lowest when they were arrested in the G2/M phase by nocodazole. This finding may improve the understanding of AD pathology and provide a new tool for anti-dementia drug development.

Keywords: Alzheimer's disease; Cell cycle; Gold nanoparticle.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alzheimer Disease / diagnosis
Alzheimer Disease / metabolism
Alzheimer Disease / pathology*
Amyloid beta-Peptides / analysis*
Amyloid beta-Peptides / metabolism
Antimetabolites / pharmacology
Biomarkers / analysis
Biomarkers / metabolism
Biotinylation
Cell Line, Tumor
Down-Regulation / drug effects
G1 Phase* / drug effects
G2 Phase / drug effects
Gold / analysis
Gold / chemistry
Humans
Immobilized Proteins / chemistry
Immobilized Proteins / metabolism
Metal Nanoparticles / analysis*
Metal Nanoparticles / chemistry
Molecular Probes / analysis
Molecular Probes / chemistry
Molecular Probes / metabolism
Nerve Tissue Proteins / analysis*
Nerve Tissue Proteins / metabolism
Neurons / chemistry
Neurons / cytology
Neurons / drug effects
Neurons / pathology*
Peptide Fragments / analysis
Peptide Fragments / metabolism
Streptavidin / chemistry
Streptavidin / metabolism
Surface Properties
Tubulin Modulators / pharmacology
Up-Regulation* / drug effects

Substances

Amyloid beta-Peptides
Antimetabolites
Biomarkers
Immobilized Proteins
Molecular Probes
Nerve Tissue Proteins
Peptide Fragments
Tubulin Modulators
amyloid beta-protein (1-16)
Gold
Streptavidin