Identification of clinically relevant Corynebacterium strains by Api Coryne, MALDI-TOF-mass spectrometry and molecular approaches

Pathol Biol (Paris). 2015 Sep;63(4-5):153-7. doi: 10.1016/j.patbio.2015.07.007. Epub 2015 Aug 20.

Abstract

We evaluated the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) for the identification of 97 Corynebacterium clinical in comparison to identification strains by Api Coryne and MALDI-TOF-MS using 16S rRNA gene and hypervariable region of rpoB genes sequencing as a reference method. C. striatum was the predominant species isolated followed by C. amycolatum. There was an agreement between Api Coryne strips and MALDI-TOF-MS identification in 88.65% of cases. MALDI-TOF-MS was unable to differentiate C. aurimucosum from C. minutissimum and C. minutissimum from C. singulare but reliably identify 92 of 97 (94.84%) strains. Two strains remained incompletely identified to the species level by MALDI-TOF-MS and molecular approaches. They belonged to Cellulomonas and Pseudoclavibacter genus. In conclusion, MALDI-TOF-MS is a rapid and reliable method for the identification of Corynebacterium species. However, some limits have been noted and have to be resolved by the application of molecular methods.

Keywords: 16S rRNA gene sequencing; 16S rRNA; Api Coryne; Corynebacterium; Corynebacterium spp.; Identification; MALDI-TOF-MS; SM-MALDI-TOF; Séquençage; rpoB.

Publication types

  • Validation Study

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Typing Techniques / methods*
  • Cellulomonas / classification
  • Cellulomonas / genetics
  • Cellulomonas / isolation & purification
  • Corynebacterium / classification*
  • Corynebacterium / genetics
  • Corynebacterium / isolation & purification
  • Corynebacterium Infections / microbiology
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / genetics
  • DNA-Directed RNA Polymerases / genetics
  • Humans
  • Micrococcaceae / classification
  • Micrococcaceae / genetics
  • Micrococcaceae / isolation & purification
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 16S / genetics
  • Reagent Strips
  • Ribotyping
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Bacterial
  • RNA, Ribosomal, 16S
  • Reagent Strips
  • DNA-Directed RNA Polymerases
  • RNA polymerase beta subunit