Pre-organized Guide RNA in the Cas9 Complex Is Ready for the Selection of Target Double-Stranded DNA

Chembiochem. 2015 Nov 2;16(16):2273-5. doi: 10.1002/cbic.201500424. Epub 2015 Sep 9.


The 3 D structure of Cas9 bound with sgRNA was solved by X-ray crystal-structure analysis. The conformation change in SpyCas9 upon binding to sgRNA changes Cas9 to target-DNA-recognition mode in which seed segments in sgRNA are pre-ordered in an A-type conformation.

Keywords: CRISPR/Cas; DNA silencing; crystal structures; genome editing; seed RNA.

MeSH terms

  • CRISPR-Cas Systems / genetics*
  • Crystallography, X-Ray
  • DNA / chemistry
  • DNA / metabolism*
  • Gene Silencing
  • Nucleic Acid Conformation
  • Protein Structure, Tertiary
  • RNA, Guide / chemistry
  • RNA, Guide / metabolism*


  • RNA, Guide
  • DNA