Clinical evaluation of a helicase-dependant amplification (HDA)-based commercial assay for the simultaneous detection of HSV-1 and HSV-2

Jeanette W P Teo; Donald Chiang; Roland Jureen; Raymond T P Lin

doi:10.1016/j.diagmicrobio.2015.07.018

Clinical evaluation of a helicase-dependant amplification (HDA)-based commercial assay for the simultaneous detection of HSV-1 and HSV-2

Diagn Microbiol Infect Dis. 2015 Nov;83(3):261-2. doi: 10.1016/j.diagmicrobio.2015.07.018. Epub 2015 Jul 28.

Authors

Jeanette W P Teo¹, Donald Chiang², Roland Jureen², Raymond T P Lin²

Affiliations

¹ Division of Microbiology, Department of Laboratory Medicine, National University Hospital, Singapore 119074, Republic of Singapore. Electronic address: Jeanette_Teo@nuhs.edu.sg.
² Division of Microbiology, Department of Laboratory Medicine, National University Hospital, Singapore 119074, Republic of Singapore.

PMID: 26302856
DOI: 10.1016/j.diagmicrobio.2015.07.018

Abstract

In this study, we evaluate the performance of a commercial assay, the AmpliVue HSV 1+2 Assay (Quidel), which employs HDA for the detection of both HSV-1 and HSV-2. The assay was tested on 307 clinical specimens (genital, oral, and dermal). When compared to shell vial virus culture and immunofluorescence typing of HSV, the positive percent agreement and negative percent agreement values were 98.2% and 90.9%, respectively. Excellent assay performance was demonstrated.

Keywords: Helicase-dependant amplification; Herpesviridae; Molecular diagnostics.

Publication types

Comparative Study
Evaluation Study

MeSH terms

DNA Helicases / metabolism*
Herpesviridae Infections / diagnosis*
Herpesvirus 1, Human / genetics
Herpesvirus 1, Human / isolation & purification*
Herpesvirus 2, Human / genetics
Herpesvirus 2, Human / isolation & purification*
Humans
Nucleic Acid Amplification Techniques / methods*

Substances

DNA Helicases