Molecular mechanism of dietary phospholipid requirement of Atlantic salmon, Salmo salar, fry

Biochim Biophys Acta. 2015 Nov;1851(11):1428-41. doi: 10.1016/j.bbalip.2015.08.006. Epub 2015 Aug 21.

Abstract

The phospholipid (PL) requirement in fish is revealed by enhanced performance when larvae are provided PL-enriched diets. To elucidate the molecular mechanism underlying PL requirement in Atlantic salmon, Salmo salar, were fed a minimal PL diet and tissue samples from major lipid metabolic sites were dissected from fry and parr. In silico analysis and cloning techniques demonstrated that salmon possess a full set of enzymes for the endogenous production of PL. The gene expression data indicated that major PL biosynthetic genes of phosphatidylcholine (PtdCho), phosphatidylethanolamine (PtdEtn) and phosphatidylinositol (PtdIns) display lower expression in intestine during the early developmental stage (fry). This is consistent with the hypothesis that the intestine of salmon is immature at the early developmental stage with limited capacity for endogenous PL biosynthesis. The results also indicate that intact PtdCho, PtdEtn and PtdIns are required in the diet at this stage. PtdCho and sphingomyelin constitute the predominant PL in chylomicrons, involved in the transport of dietary lipids from the intestine to the rest of the body. As sphingomyelin can be produced from PtdCho in intestine of fry, our findings suggest that supplementation of dietary PtdCho alone during early developmental stages of Atlantic salmon would be sufficient to promote chylomicron formation. This would support efficient transport of dietary lipids, including PL precursors, from the intestine to the liver where biosynthesis of PtdEtn, PtdSer, and PtdIns is not compromised as in intestine facilitating efficient utilisation of dietary energy and the endogenous production of membrane PL for the rapidly growing and developing animal.

Keywords: Atlantic salmon; Dietary requirement; Gene expression; Phosphoglycerides; Phospholipids; qPCR.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Biological Transport
  • CDPdiacylglycerol-Serine O-Phosphatidyltransferase / genetics
  • CDPdiacylglycerol-Serine O-Phosphatidyltransferase / metabolism*
  • Chylomicrons / biosynthesis
  • Dietary Fats / administration & dosage
  • Dietary Fats / metabolism*
  • Fish Proteins / genetics
  • Fish Proteins / metabolism*
  • Gene Expression Regulation, Developmental
  • Intestinal Mucosa / metabolism
  • Intestines / growth & development
  • Larva / genetics
  • Larva / growth & development
  • Larva / metabolism
  • Liver / growth & development
  • Liver / metabolism
  • Molecular Sequence Annotation
  • Molecular Sequence Data
  • Phosphatidylcholines / biosynthesis
  • Phosphatidylethanolamines / biosynthesis
  • Phosphatidylinositols / biosynthesis
  • Salmo salar / genetics
  • Salmo salar / growth & development
  • Salmo salar / metabolism*
  • Sequence Alignment
  • Sphingomyelins / biosynthesis

Substances

  • Chylomicrons
  • Dietary Fats
  • Fish Proteins
  • Phosphatidylcholines
  • Phosphatidylethanolamines
  • Phosphatidylinositols
  • Sphingomyelins
  • phosphatidylethanolamine
  • CDPdiacylglycerol-Serine O-Phosphatidyltransferase