Bacillus gossypii sp. nov., isolated from the stem of Gossypium hirsutum

Int J Syst Evol Microbiol. 2015 Nov;65(11):4163-4168. doi: 10.1099/ijsem.0.000555. Epub 2015 Aug 24.

Abstract

A Gram-stain-positive, facultatively anaerobic, endospore-forming organism, isolated from the stem of Gossypium hirsutum, was studied to determine its taxonomic position. On the basis of 16S rRNA gene sequence similarity comparisons, strain JM-267T was grouped in the genus Bacillus, related most closely to the type strains of Bacillus simplex and Bacillus huizhouensis (both 97.8%), Bacillus muralis (97.7%), Bacillus butanolivorans and Bacillus psychrosaccharolyticus (both 97.3%). 16S rRNA gene sequence similarity to the sequences of the type strains of other Bacillus species was < 97.0%. The fatty acid profile supported the grouping of the strain to the genus Bacillus. As major fatty acids, anteiso-C15:0, iso-C15:0, iso-C14:0 and iso-C16:0 were detected. The polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major quinone was menaquinone 7 (MK-7). DNA-DNA hybridizations with B. simplex DSM 1321T, B. huizhouensis GSS03T, B. muralis LMG 20238T, B. butanolivorans LMG 23974T and B. psychrosaccharolyticus DSM 6T resulted in values clearly below 70%. In addition, physiological and biochemical test results allowed the clear phenotypic differentiation of strain JM-267T from the most closely related species. Hence, strain JM-267T is considered to represent a novel species of the genus Bacillus, for which the name Bacillus gossypii sp. nov. is proposed. The type strain is JM-267T ( = DSM 100034T = LMG 28742T).

MeSH terms

  • Alabama
  • Bacillus / classification*
  • Bacillus / genetics
  • Bacillus / isolation & purification
  • Bacterial Typing Techniques
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Gossypium / microbiology*
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Phospholipids / chemistry
  • Phylogeny*
  • Plant Stems / microbiology*
  • Polyamines / chemistry
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Vitamin K 2 / analogs & derivatives
  • Vitamin K 2 / chemistry

Substances

  • DNA, Bacterial
  • Fatty Acids
  • Phospholipids
  • Polyamines
  • RNA, Ribosomal, 16S
  • Vitamin K 2
  • vitamin MK 7