Anti-Pseudomonas aeruginosa IgY antibodies augment bacterial clearance in a murine pneumonia model

J Cyst Fibros. 2016 Mar;15(2):171-8. doi: 10.1016/j.jcf.2015.08.002. Epub 2015 Aug 21.


Background: Oral prophylactic therapy by gargling with pathogen-specific egg yolk immunoglobulins (IgY) may reduce the initial airway colonization with Pseudomonas aeruginosa in cystic fibrosis (CF) patients. IgY antibodies impart passive immunization and we investigated the effects of anti-P. aeruginosa IgY antibodies on bacterial eradication in a murine pneumonia model.

Methods: P. aeruginosa pneumonia was established in Balb/c mice and the effects of prophylactic IgY administration on lung bacteriology, clinical parameters and subsequent inflammation were compared to controls.

Results: Prophylactic administration of IgY antibodies targeting P. aeruginosa significantly reduced the bacterial burden by 2-log 24h post-infection compared to controls and was accompanied by significantly reduced clinical symptom scores and successive inflammatory cytokine profile indicative of diminished lung inflammation.

Conclusions: Passive immunization by anti-P. aeruginosa IgY therapy facilitates promptly bacterial clearance and moderates inflammation in P. aeruginosa lung infection and may serve as an adjunct to antibiotics in reducing early colonization.

Keywords: Cystic fibrosis; IgY; Immunotherapy; Pseudomonas aeruginosa.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Animals
  • Antibodies, Anti-Idiotypic / immunology
  • Antibodies, Anti-Idiotypic / therapeutic use*
  • Antibodies, Bacterial / immunology
  • Antibodies, Bacterial / therapeutic use*
  • Disease Models, Animal
  • Female
  • Immunoglobulins / immunology
  • Immunoglobulins / therapeutic use*
  • Mice
  • Mice, Inbred BALB C
  • Microbial Viability
  • Pneumonia, Bacterial / drug therapy*
  • Pneumonia, Bacterial / immunology
  • Pneumonia, Bacterial / microbiology
  • Pseudomonas Infections / drug therapy*
  • Pseudomonas Infections / immunology
  • Pseudomonas Infections / microbiology
  • Pseudomonas aeruginosa / drug effects
  • Pseudomonas aeruginosa / immunology*
  • Pseudomonas aeruginosa / isolation & purification


  • Antibodies, Anti-Idiotypic
  • Antibodies, Bacterial
  • IgY
  • Immunoglobulins