Detection of adulterated murine components in meat products by TaqMan© real-time PCR

Xin Fang; Chi Zhang

doi:10.1016/j.foodchem.2015.07.020

Detection of adulterated murine components in meat products by TaqMan© real-time PCR

Food Chem. 2016 Feb 1:192:485-90. doi: 10.1016/j.foodchem.2015.07.020. Epub 2015 Jul 8.

Authors

Xin Fang¹, Chi Zhang²

Affiliations

¹ National Center of Supervision & Inspection on Processed Food and Food Additives Quality, Nanjing Institute of Product Quality Inspection, No. 3 Jialingjiang East Street, 210019 Nanjing, China.
² National Center of Supervision & Inspection on Processed Food and Food Additives Quality, Nanjing Institute of Product Quality Inspection, No. 3 Jialingjiang East Street, 210019 Nanjing, China; National Key Laboratory of Bioelectronics, Southeast University, No. 2 Sipai Lou, 210096 Nanjing, China. Electronic address: cavy78@gmail.com.

PMID: 26304376
DOI: 10.1016/j.foodchem.2015.07.020

Abstract

Using murine meat to substitute mutton has been identified as a new type of meat fraud in China, yet no detection method for murine species has been reported. Here, three kinds of rodent were used as target species to establish a murine-specific real-time PCR method of detection. The mitochondrial cytochrome b gene (cytb) of each target was sequenced and a TaqMan probe was designed based on the cytb. Simultaneously, an internal positive control (IPC) plasmid along with its respective probe were designed to monitor the PCR reaction. As a result, the duplex real-time PCR system was verified to be specific. The limit of detection (LOD) was lower than 1 pg of DNA per reaction and 0.1% murine contamination in meat mixtures. Standard curves were generated for a quantitative analysis. Thus, this study provided a new tool to control the quality of meat products for official and third-party laboratories.

Keywords: Adulteration; Meat products; Murine meat; Real-time PCR.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
China
Cytochromes b / genetics
DNA / analysis
Food Contamination / analysis*
Food Quality
Fraud
Meat / analysis*
Meat Products / analysis*
Mice
Plasmids
Rats
Real-Time Polymerase Chain Reaction / methods*

Substances

DNA
Cytochromes b