Characterization of Inulin Hydrolyzing Enzyme(s) in Oleaginous Yeast Trichosporon cutaneum in Consolidated Bioprocessing of Microbial Lipid Fermentation

Juan Wang; Huizhan Zhang; Jie Bao

doi:10.1007/s12010-015-1798-5

Characterization of Inulin Hydrolyzing Enzyme(s) in Oleaginous Yeast Trichosporon cutaneum in Consolidated Bioprocessing of Microbial Lipid Fermentation

Appl Biochem Biotechnol. 2015 Nov;177(5):1083-98. doi: 10.1007/s12010-015-1798-5. Epub 2015 Aug 26.

Authors

Juan Wang¹, Huizhan Zhang², Jie Bao³

Affiliations

¹ State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
² State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China. huizhzh@ecust.edu.cn.
³ State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China. jbao@ecust.edu.cn.

PMID: 26306527
DOI: 10.1007/s12010-015-1798-5

Abstract

Oleaginous yeast Trichosporon cutaneum CGMCC 2.1374 was found to utilize inulin directly for microbial lipid fermentation without a hydrolysis step. The potential inulinase-like enzyme(s) in T. cutaneum CGMCC 2.1374 were characterized and compared with other inulinase enzymes produced by varied yeast strains. The consolidated bioprocessing (CBP) for lipid accumulated using inulin was optimized with 4.79 g/L of lipid produced from 50 g/L inulin with the lipid content of 33.6% in dry cells. The molecular weight of the enzyme was measured which was close to invertase in Saccharomyces cerevisiae. The study provided information for inulin hydrolyzing enzyme(s) in oleaginous yeasts, as well as a preliminary CBP process for lipid production from inulin feedstock.

Keywords: Consolidated bioprocessing; Dry cell weight; Inulin hydrolyzing enzyme; Lipid production; Trichosporon cutaneum.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Fungal Proteins / chemistry*
Glycoside Hydrolases / chemistry*
Hydrolysis
Lipids / chemistry*
Trichosporon / enzymology*

Substances

Fungal Proteins
Lipids
Glycoside Hydrolases
inulinase