Hypoperfusion of the Adventitial Vasa Vasorum Develops an Abdominal Aortic Aneurysm

Hiroki Tanaka; Nobuhiro Zaima; Takeshi Sasaki; Masaki Sano; Naoto Yamamoto; Takaaki Saito; Kazunori Inuzuka; Takahiro Hayasaka; Naoko Goto-Inoue; Yuki Sugiura; Kohji Sato; Hirona Kugo; Tatsuya Moriyama; Hiroyuki Konno; Mitsutoshi Setou; Naoki Unno

doi:10.1371/journal.pone.0134386

Hypoperfusion of the Adventitial Vasa Vasorum Develops an Abdominal Aortic Aneurysm

PLoS One. 2015 Aug 26;10(8):e0134386. doi: 10.1371/journal.pone.0134386. eCollection 2015.

Authors

Hiroki Tanaka¹, Nobuhiro Zaima², Takeshi Sasaki³, Masaki Sano⁴, Naoto Yamamoto⁴, Takaaki Saito⁴, Kazunori Inuzuka⁴, Takahiro Hayasaka⁵, Naoko Goto-Inoue⁶, Yuki Sugiura⁷, Kohji Sato³, Hirona Kugo⁸, Tatsuya Moriyama⁸, Hiroyuki Konno⁹, Mitsutoshi Setou¹⁰, Naoki Unno⁴

Affiliations

¹ Division of Vascular Surgery, Hamamatsu University School of Medicine, Hamamatsu, Japan; Department of Cell Biology and Anatomy, Hamamatsu University School of Medicine, Hamamatsu, Japan; Second Department of Surgery, Hamamatsu University School of Medicine, Hamamatsu, Japan.
² Department of Cell Biology and Anatomy, Hamamatsu University School of Medicine, Hamamatsu, Japan; Department of Applied Biological Chemistry, Graduate School of Agriculture, Kinki University, Nara, Japan.
³ Department of Anatomy and Neuroscience, Hamamatsu University School of Medicine, Hamamatsu, Japan.
⁴ Division of Vascular Surgery, Hamamatsu University School of Medicine, Hamamatsu, Japan; Second Department of Surgery, Hamamatsu University School of Medicine, Hamamatsu, Japan.
⁵ Department of Cell Biology and Anatomy, Hamamatsu University School of Medicine, Hamamatsu, Japan; Health Innovation & Technology Center, Hokkaido University, Sapporo, Japan.
⁶ Department of Cell Biology and Anatomy, Hamamatsu University School of Medicine, Hamamatsu, Japan; Department of Health Promotion Science, Tokyo Metropolitan University, Tokyo, Japan.
⁷ Department of Cell Biology and Anatomy, Hamamatsu University School of Medicine, Hamamatsu, Japan; Department of Biochemistry, School of Medicine, Keio University, Tokyo, Japan.
⁸ Department of Applied Biological Chemistry, Graduate School of Agriculture, Kinki University, Nara, Japan.
⁹ Second Department of Surgery, Hamamatsu University School of Medicine, Hamamatsu, Japan.
¹⁰ Department of Cell Biology and Anatomy, Hamamatsu University School of Medicine, Hamamatsu, Japan.

Abstract

The aortic wall is perfused by the adventitial vasa vasorum (VV). Tissue hypoxia has previously been observed as a manifestation of enlarged abdominal aortic aneurysms (AAAs). We sought to determine whether hypoperfusion of the adventitial VV could develop AAAs. We created a novel animal model of adventitial VV hypoperfusion with a combination of a polyurethane catheter insertion and a suture ligation of the infrarenal abdominal aorta in rats. VV hypoperfusion caused tissue hypoxia and developed infrarenal AAA, which had similar morphological and pathological characteristics to human AAA. In human AAA tissue, the adventitial VV were stenotic in both small AAAs (30-49 mm in diameter) and in large AAAs (> 50 mm in diameter), with the sac tissue in these AAAs being ischemic and hypoxic. These results indicate that hypoperfusion of adventitial VV has critical effects on the development of infrarenal AAA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aged
Animals
Aorta, Abdominal / metabolism
Aorta, Abdominal / pathology
Aorta, Abdominal / physiopathology
Aortic Aneurysm, Abdominal / complications
Aortic Aneurysm, Abdominal / metabolism
Aortic Aneurysm, Abdominal / pathology
Aortic Aneurysm, Abdominal / physiopathology*
Blood Circulation*
Constriction, Pathologic / complications
Female
Humans
Male
Oxygen / metabolism
Rats
Rats, Sprague-Dawley
Vasa Vasorum / metabolism
Vasa Vasorum / pathology
Vasa Vasorum / physiopathology*

Substances

Oxygen

Grants and funding

This work was supported by Grants-in-Aid for Scientific Research (B) (20291958) to NU, (C) (26462103) to KI; Grants-in-Aid for Young Scientists (A) (25713024) to NZ; a Grant-in-Aid for SENTAN from the Japan Science and Technology Agency (JST); Tokutei Lipid Machinery; and Young Scientists S (2067004) to M. Setou. Financial support for overseas study was provided by the Japan Research Foundation for Clinical Pharmacology to HT.