Simple wavefront correction framework for two-photon microscopy of in-vivo brain

Biomed Opt Express. 2015 Jul 23;6(8):2997-3013. doi: 10.1364/BOE.6.002997. eCollection 2015 Aug 1.


We present an easily implemented wavefront correction scheme that has been specifically designed for in-vivo brain imaging. The system can be implemented with a single liquid crystal spatial light modulator (LCSLM), which makes it compatible with existing patterned illumination setups, and provides measurable signal improvements even after a few seconds of optimization. The optimization scheme is signal-based and does not require exogenous guide-stars, repeated image acquisition or beam constraint. The unconstrained beam approach allows the use of Zernike functions for aberration correction and Hadamard functions for scattering correction. Low order corrections performed in mouse brain were found to be valid up to hundreds of microns away from the correction location.

Keywords: (010.1080) Active or adaptive optics; (170.2520) Fluorescence microscopy; (170.5810) Scanning microscopy.