Lipid-bilayer-assisted two-dimensional self-assembly of DNA origami nanostructures

Nat Commun. 2015 Aug 27;6:8052. doi: 10.1038/ncomms9052.


Self-assembly is a ubiquitous approach to the design and fabrication of novel supermolecular architectures. Here we report a strategy termed 'lipid-bilayer-assisted self-assembly' that is used to assemble DNA origami nanostructures into two-dimensional lattices. DNA origami structures are electrostatically adsorbed onto a mica-supported zwitterionic lipid bilayer in the presence of divalent cations. We demonstrate that the bilayer-adsorbed origami units are mobile on the surface and self-assembled into large micrometre-sized lattices in their lateral dimensions. Using high-speed atomic force microscopy imaging, a variety of dynamic processes involved in the formation of the lattice, such as fusion, reorganization and defect filling, are successfully visualized. The surface modifiability of the assembled lattice is also demonstrated by in situ decoration with streptavidin molecules. Our approach provides a new strategy for preparing versatile scaffolds for nanofabrication and paves the way for organizing functional nanodevices in a micrometer space.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aluminum Silicates
  • DNA*
  • Lipid Bilayers*
  • Microscopy, Atomic Force
  • Nanostructures*
  • Nanotechnology
  • Nucleic Acid Conformation*
  • Static Electricity


  • Aluminum Silicates
  • Lipid Bilayers
  • DNA
  • mica