Involvement of Antizyme Characterized from the Small Abalone Haliotis diversicolor in Gonadal Development

PLoS One. 2015 Aug 27;10(8):e0135251. doi: 10.1371/journal.pone.0135251. eCollection 2015.

Abstract

The small abalone Haliotis diversicolor is an economically important mollusk that is widely cultivated in Southern China. Gonad precocity may affect the aquaculture of small abalone. Polyamines, which are small cationic molecules essential for cellular proliferation, may affect gonadal development. Ornithine decarboxylase (ODC) and antizyme (AZ) are essential elements of a feedback circuit that regulates cellular polyamines. This paper presents the molecular cloning and characterization of AZ from small abalone. Sequence analysis showed that the cDNA sequence of H. diversicolor AZ (HdiODCAZ) consisted of two overlapping open reading frames (ORFs) and conformed to the +1 frameshift property of the frame. Thin Layer chromatography (TLC) analysis suggested that the expressed protein encoded by +1 ORF2 was the functional AZ that targets ODC to 26S proteasome degradation. The result demonstrated that the expression level of AZ was higher than that of ODC in the ovary of small abalone. In addition, the expression profiles of ODC and AZ at the different development stages of the ovary indicated that these two genes might be involved in the gonadal development of small abalone.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Proliferation / genetics
  • Cloning, Molecular
  • Female
  • Gastropoda / enzymology
  • Gastropoda / growth & development*
  • Gene Expression Regulation, Developmental
  • Molecular Sequence Data
  • Open Reading Frames
  • Ovary / metabolism
  • Polyamines / metabolism
  • Protein Structure, Tertiary
  • Proteins / physiology*
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Analysis, Protein

Substances

  • Polyamines
  • Proteins
  • ornithine decarboxylase antizyme

Grants and funding

This work was financially supported by National Natural Science Foundation of China (31160528 and U1205121), National High Technology Research and Development Program of China (2012AA10A412), Earmarked Fund for Modern Agro-industry Technology Research System (CARS-48), and South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.