Generation of cancer-specific CD8(+) CD69(+) cells inhibits colon cancer growth

Immunobiology. 2016 Jan;221(1):1-5. doi: 10.1016/j.imbio.2015.08.010. Epub 2015 Aug 18.


The therapy of colon cancer (Cca) is poor currently. The abnormality of immune function is recognized in cancer-bearing hosts. This study aims to inhibit Cca growth with the in vivo induction of Cca-specific CD8(+) CD69(+) T cell polarization. In this study, a mouse tumor model was created with a mouse colon cancer (Cca) cell line. The Cca-bearing mice were immunized with Cca extracts and alum (using as an adjuvant). The CD8(+) CD69(+) cell polarization status was analyzed by flow cytometry and enzyme-linked immunosorbent assay. The results showed that a Cca antigen-specific CD8(+) CD69(+) cells were induced in the Cca-bearing mice. The Cca growth was efficiently inhibited by the Cca-specific CD8(+) CD69(+) cell response. The Cca-specific CD8(+) CD69(+) cells were detected in the Cca mass and spleen, which had the ability to induce Cca cell apoptosis. In conclusion, the Cca-specific CD8(+) CD69(+) cell polarization can be induced in Cca-bearing mice, which can efficiently inhibit the Cca growth.

Keywords: Antitumor immunity; Apoptosis; CD8 T cell; Cancer mouse model; Colon cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic / pharmacology
  • Alum Compounds / pharmacology
  • Animals
  • Antigens, CD / genetics
  • Antigens, CD / immunology
  • Antigens, Differentiation, T-Lymphocyte / genetics
  • Antigens, Differentiation, T-Lymphocyte / immunology
  • CD8 Antigens / genetics
  • CD8 Antigens / immunology
  • CD8-Positive T-Lymphocytes / cytology
  • CD8-Positive T-Lymphocytes / immunology*
  • Cancer Vaccines / pharmacology*
  • Cell Line, Tumor
  • Colonic Neoplasms / genetics
  • Colonic Neoplasms / immunology
  • Colonic Neoplasms / pathology
  • Colonic Neoplasms / therapy*
  • Complex Mixtures / pharmacology*
  • Disease Models, Animal
  • Gene Expression
  • Immunization
  • Lectins, C-Type / genetics
  • Lectins, C-Type / immunology
  • Lymphocytes, Tumor-Infiltrating / cytology
  • Lymphocytes, Tumor-Infiltrating / immunology*
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Neoplasm Transplantation
  • Spleen / immunology
  • Spleen / pathology


  • Adjuvants, Immunologic
  • Alum Compounds
  • Antigens, CD
  • Antigens, Differentiation, T-Lymphocyte
  • CD69 antigen
  • CD8 Antigens
  • Cancer Vaccines
  • Complex Mixtures
  • Lectins, C-Type
  • aluminum sulfate