A polysaccharide from cultured mycelium of Hericium erinaceus and its anti-chronic atrophic gastritis activity

Int J Biol Macromol. 2015 Nov;81:656-61. doi: 10.1016/j.ijbiomac.2015.08.043. Epub 2015 Aug 24.

Abstract

A polysaccharide named EP-1 was found by screening cultured mycelium of Hericium erinaceus, which was extracted and subjected to precipitation with ethanol, hollow-fiber ultrafiltration and ion-exchange chromatography. The polysaccharide has a molecular weight of approximately 3100Da and is composed of glucose, mannose and galactose, thus being a heteroglycan. EP-1 has a backbone of α-d-Glc(1→3) and β-d-Glc(1→3). The β-d-Glc(1→3) and α-d-Gal-(1→3) were regarded as branches attached to the C-4 position. The α-d-Man was regarded as a terminal residue. The anti-CAG activity was evaluated in experimental systems using a cell model for identification. The polysaccharide significantly inhibited the growth of MC cells obtained from human gastric mucosa epithelium (GES-1) cells transformed by MNNG, which were used as a chronic atrophic gastritis cell model. It also interfered with the MC cells by inducing cell cycle arrest. Thus, EP-1 shows potential for the development of new functional foods and drugs.

Keywords: Anti-CAG activity; Hericium erinaceus; Polysaccharide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Basidiomycota / chemistry*
  • Carbohydrates / chemistry
  • Cell Cycle Checkpoints / drug effects
  • Cell Line
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • Fungal Polysaccharides / chemistry*
  • Fungal Polysaccharides / isolation & purification
  • Fungal Polysaccharides / pharmacology*
  • Gas Chromatography-Mass Spectrometry
  • Gastritis, Atrophic / drug therapy
  • Gastritis, Atrophic / pathology*
  • Humans
  • Magnetic Resonance Spectroscopy
  • Methylation
  • Mycelium / chemistry*
  • Spectroscopy, Fourier Transform Infrared

Substances

  • Carbohydrates
  • Fungal Polysaccharides