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, 12 (9), 821-2

Removing Roadblocks to Deep Sequencing of Modified RNAs

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Removing Roadblocks to Deep Sequencing of Modified RNAs

Jeremy E Wilusz. Nat Methods.

Abstract

By removing modified nucleotides that block reverse transcriptase, two methods have now made tRNAs amenable to RNA-seq.

Conflict of interest statement

COMPETING FINANCIAL INTERESTS

The author declares no competing financial interests.

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Figure 1
Figure 1
Removing methylated nucleotides to sequence tRNAs. Whereas reverse transcriptase (RT) cannot extend through a fully modified tRNA (left), treatment with AlkB removes select methylated nucleotides to allow efficient reverse transcription and deep sequencing (center and right). Full-length and truncated cDNAs are shown in purple and green, respectively.

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