PDK1 induces JunB, EMT, cell migration and invasion in human gallbladder cancer

Oncotarget. 2015 Oct 6;6(30):29076-86. doi: 10.18632/oncotarget.4931.


The protein 3-phosphoinositide-dependent protein kinase 1 (PDK1) is upregulated in cancer. Here we showed that PDK1 stimulated cell proliferation, invasion and metastasis in gallbladder cancer (GBC), by inducing JunB and epithelial-mesenchymal transition. JunB levels were increased in GBC samples and positively correlated with PDK1 levels in tumors. High levels of JunB predicted poor overall survival in GBC patients. Thus, PDK1 functions as a tumor promoter in human GBC by upregulating JunB.

Keywords: 3-phosphoinositide-dependent protein kinase 1; JunB; epithelial–mesenchymal transition; invasion; migration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3-Phosphoinositide-Dependent Protein Kinases / genetics
  • 3-Phosphoinositide-Dependent Protein Kinases / metabolism*
  • Animals
  • Cell Line, Tumor
  • Cell Movement*
  • Epithelial-Mesenchymal Transition*
  • Female
  • Gallbladder Neoplasms / enzymology*
  • Gallbladder Neoplasms / genetics
  • Gallbladder Neoplasms / mortality
  • Gallbladder Neoplasms / pathology
  • Gene Expression Regulation, Enzymologic
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Male
  • Mice, Nude
  • Middle Aged
  • Neoplasm Invasiveness
  • Prognosis
  • Signal Transduction
  • Time Factors
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Transfection
  • Up-Regulation


  • JunB protein, human
  • Transcription Factors
  • 3-Phosphoinositide-Dependent Protein Kinases
  • PDPK1 protein, human