A comparison of Luminex xTAG® Gastrointestinal Pathogen Panel (xTAG GPP) and routine tests for the detection of enteropathogens circulating in Southern China

Diagn Microbiol Infect Dis. 2015 Nov;83(3):325-30. doi: 10.1016/j.diagmicrobio.2015.07.024. Epub 2015 Jul 30.

Abstract

To investigate whether Luminex xTAG® Gastrointestinal Pathogen Panel (xTAG GPP) is applicable for the diagnosis of diarrhea and surveillance of enteropathogens circulating in Southern China, 290 stool samples were tested for 15 kinds of enteropathogens using xTAG GPP and compared to the results from the routine tests, including culture; immunochromatography; real-time PCR; microscopy; and a third method, gene sequencing. One hundred fifty-nine samples were positive, yielding a total of 181 enteropathogens (69 bacteria and 112 viruses), with rotavirus being most prevalent (39.0%, 62/159). The overall sensitivity and specificity of xTAG GPP were 96.3% (93.3-98.2%) and 99.8% (99.6-99.9%), respectively, with a combination of the methods as the gold standard. The coinfection rates detected by the routine tests and xTAG GPP were 10.0% (25 double and 4 triple infections) and 12.1% (29 double, 4 triple and 2 quadruple infections), respectively. xTAG GPP is a powerful tool for the identification of multiple enteropathogens.

Keywords: Comparative evaluation; Multiple enteropathogens identification; xTAG GPP.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Bacteria / classification
  • Bacteria / isolation & purification
  • Bacterial Infections / diagnosis*
  • Child
  • Child, Preschool
  • China
  • Diarrhea / diagnosis*
  • Feces / microbiology
  • Feces / virology
  • Female
  • Gastroenteritis / diagnosis*
  • Humans
  • Infant
  • Infant, Newborn
  • Male
  • Microbiological Techniques / methods*
  • Middle Aged
  • Molecular Diagnostic Techniques / methods*
  • Prospective Studies
  • Sensitivity and Specificity
  • Virus Diseases / diagnosis*
  • Viruses / classification
  • Viruses / isolation & purification
  • Young Adult