Lil3 dimerization and chlorophyll binding in Arabidopsis thaliana

FEBS Lett. 2015 Oct 7;589(20 Pt B):3064-70. doi: 10.1016/j.febslet.2015.08.023. Epub 2015 Aug 29.

Abstract

The two-helix light harvesting like (Lil) protein Lil3 belongs to the family of chlorophyll binding light harvesting proteins of photosynthetic membranes. A function in tetrapyrrol synthesis and stabilization of geranylgeraniol reductase has been shown. Lil proteins contain the chlorophyll a/b-binding motif; however, binding of chlorophyll has not been demonstrated. We find that Lil3.2 from Arabidopsis thaliana forms heterodimers with Lil3.1 and binds chlorophyll. Lil3.2 heterodimerization (25±7.8 nM) is favored relative to homodimerization (431±59 nM). Interaction of Lil3.2 with chlorophyll a (231±49 nM) suggests that heterodimerization precedes binding of chlorophyll in Arabidopsis thaliana.

Keywords: Chlorophyll a; Hydrophobic cluster analysis plot; Light harvesting like protein 3; Microscale thermophoresis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Amino Acids / chemistry
  • Amino Acids / genetics
  • Amino Acids / metabolism
  • Arabidopsis / genetics
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / chemistry
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Binding Sites / genetics
  • Chlorophyll / metabolism*
  • Chloroplast Proteins / chemistry
  • Chloroplast Proteins / genetics
  • Chloroplast Proteins / metabolism*
  • Electrophoresis, Polyacrylamide Gel
  • Kinetics
  • Light-Harvesting Protein Complexes / chemistry
  • Light-Harvesting Protein Complexes / genetics
  • Light-Harvesting Protein Complexes / metabolism
  • Molecular Sequence Data
  • Protein Binding
  • Protein Multimerization
  • Surface Plasmon Resonance

Substances

  • Amino Acids
  • Arabidopsis Proteins
  • Chloroplast Proteins
  • Light-Harvesting Protein Complexes
  • light-harvesting-like protein 3, Arabidopsis
  • Chlorophyll