Magnetic NGF-releasing PLLA/iron oxide nanoparticles direct extending neurites and preferentially guide neurites along aligned electrospun microfibers

ACS Chem Neurosci. 2015 Nov 18;6(11):1781-8. doi: 10.1021/acschemneuro.5b00189. Epub 2015 Sep 3.


Nerve growth factor releasing composite nanoparticles (NGF-cNPs) were developed to direct the extension of neurite outgrowth from dorsal root ganglia (DRG). Iron oxide magnetic nanoparticles were incorporated into poly-l-lactic acid (PLLA) nanoparticles in order to position the NGF-cNPs in a culture dish. Neurites growing from DRG extended toward the NGF released from the NGF-cNPs. DRG were then cultured on aligned PLLA microfibers in the presence of NGF-cNPs, and these biomaterials combined to align DRG neurite extension along one axis and preferentially toward the NGF-cNPs. This combinatorial biomaterial approach shows promise as a strategy to direct the extension of regenerating neurites.

Keywords: Poly-l-lactic acid; electrospun fiber; magnetic nanoparticle; nerve growth factor; neurite extension.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cell Enlargement / drug effects
  • Chick Embryo
  • Drug Delivery Systems / instrumentation
  • Drug Delivery Systems / methods*
  • Ferric Compounds / chemistry
  • Ganglia, Spinal / drug effects
  • Ganglia, Spinal / physiology
  • Immunohistochemistry
  • Lactic Acid / chemistry
  • Lumbar Vertebrae
  • Magnetite Nanoparticles* / chemistry
  • Microscopy, Electron, Scanning
  • Nerve Growth Factor / administration & dosage*
  • Nerve Growth Factor / pharmacokinetics
  • Neurites / drug effects*
  • Neurites / physiology
  • Neuroprotective Agents / administration & dosage*
  • Neuroprotective Agents / pharmacokinetics
  • Polyesters
  • Polymers / chemistry
  • Tissue Culture Techniques / methods


  • Ferric Compounds
  • Magnetite Nanoparticles
  • Neuroprotective Agents
  • Polyesters
  • Polymers
  • ferric oxide
  • Lactic Acid
  • poly(lactide)
  • Nerve Growth Factor