Abstract
Nerve growth factor releasing composite nanoparticles (NGF-cNPs) were developed to direct the extension of neurite outgrowth from dorsal root ganglia (DRG). Iron oxide magnetic nanoparticles were incorporated into poly-l-lactic acid (PLLA) nanoparticles in order to position the NGF-cNPs in a culture dish. Neurites growing from DRG extended toward the NGF released from the NGF-cNPs. DRG were then cultured on aligned PLLA microfibers in the presence of NGF-cNPs, and these biomaterials combined to align DRG neurite extension along one axis and preferentially toward the NGF-cNPs. This combinatorial biomaterial approach shows promise as a strategy to direct the extension of regenerating neurites.
Keywords:
Poly-l-lactic acid; electrospun fiber; magnetic nanoparticle; nerve growth factor; neurite extension.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Cell Enlargement / drug effects
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Chick Embryo
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Drug Delivery Systems / instrumentation
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Drug Delivery Systems / methods*
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Ferric Compounds / chemistry
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Ganglia, Spinal / drug effects
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Ganglia, Spinal / physiology
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Immunohistochemistry
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Lactic Acid / chemistry
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Lumbar Vertebrae
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Magnetite Nanoparticles* / chemistry
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Microscopy, Electron, Scanning
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Nerve Growth Factor / administration & dosage*
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Nerve Growth Factor / pharmacokinetics
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Neurites / drug effects*
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Neurites / physiology
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Neuroprotective Agents / administration & dosage*
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Neuroprotective Agents / pharmacokinetics
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Polyesters
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Polymers / chemistry
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Tissue Culture Techniques / methods
Substances
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Ferric Compounds
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Magnetite Nanoparticles
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Neuroprotective Agents
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Polyesters
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Polymers
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ferric oxide
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Lactic Acid
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poly(lactide)
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Nerve Growth Factor