Whole-genome sequencing identifies emergence of a quinolone resistance mutation in a case of Stenotrophomonas maltophilia bacteremia

Antimicrob Agents Chemother. 2015 Nov;59(11):7117-20. doi: 10.1128/AAC.01723-15. Epub 2015 Aug 31.


Whole-genome sequences for Stenotrophomonas maltophilia serial isolates from a bacteremic patient before and after development of levofloxacin resistance were assembled de novo and differed by one single-nucleotide variant in smeT, a repressor for multidrug efflux operon smeDEF. Along with sequenced isolates from five contemporaneous cases, they displayed considerable diversity compared against all published complete genomes. Whole-genome sequencing and complete assembly can conclusively identify resistance mechanisms emerging in S. maltophilia strains during clinical therapy.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA, Bacterial / genetics
  • Drug Resistance, Multiple, Bacterial / genetics
  • Genome, Bacterial / genetics*
  • Gram-Negative Bacterial Infections / microbiology*
  • Microbial Sensitivity Tests
  • Mutation
  • Quinolones / pharmacology*
  • Stenotrophomonas maltophilia / immunology*


  • DNA, Bacterial
  • Quinolones

Supplementary concepts

  • Stenotrophomonas maltophilia bacteremia

Associated data

  • GENBANK/CP011010
  • GENBANK/CP011305
  • GENBANK/CP011306
  • GENBANK/JZIU00000000
  • GENBANK/JZIU01000000
  • GENBANK/JZIV00000000
  • GENBANK/JZIV01000000
  • GENBANK/JZIW00000000
  • GENBANK/JZIW01000000
  • GENBANK/JZTX00000000
  • GENBANK/JZTX01000000