High-resolution analysis of the human T-cell receptor repertoire

Nat Commun. 2015 Sep 1;6:8081. doi: 10.1038/ncomms9081.

Abstract

Unbiased dissection of T-cell receptor (TCR) repertoire diversity at the nucleotide level could provide important insights into human immunity. Here we show that TCR ligation-anchored-magnetically captured PCR (TCR-LA-MC PCR) identifies TCR α- and β-chain diversity without sequence-associated or quantitative restrictions in healthy and diseased conditions. TCR-LA-MC PCR identifies convergent recombination events, classifies different stages of cutaneous T-cell lymphoma in vivo and demonstrates TCR reactivation after in vitro cytomegalovirus stimulation. TCR-LA-MC PCR allows ultra-deep data access to both physiological TCR diversity and mechanisms influencing clonality in all clinical settings with restricted or distorted TCR repertoires.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Animals
  • Cytomegalovirus Infections / genetics*
  • Female
  • Flow Cytometry
  • Genes, T-Cell Receptor alpha / genetics*
  • Genes, T-Cell Receptor beta / genetics*
  • Genetic Variation
  • High-Throughput Nucleotide Sequencing
  • Humans
  • In Vitro Techniques
  • Jurkat Cells
  • Lymphoma, T-Cell, Cutaneous / genetics
  • Male
  • Mice
  • Middle Aged
  • Polymerase Chain Reaction
  • Receptors, Antigen, T-Cell / genetics
  • Receptors, Antigen, T-Cell, alpha-beta / genetics*
  • Sezary Syndrome / genetics*
  • Skin Neoplasms / genetics*

Substances

  • Receptors, Antigen, T-Cell
  • Receptors, Antigen, T-Cell, alpha-beta

Associated data

  • SRA/SRP059581