Six variable supplementations of thiamine magnesium, and sulfates were given to 30 male adult rats in their diets. After 3 weeks, the concentrations of thiamine in the blood and liver and those of cholesterol, phospholipids, and triglycerides in the serum were determined. Blood thiamine level did not reflect the vitamin content in liver. Sufficient and/or excessive intake of the 3 supplementations caused an increase in liver thiamine content and body weight gain; it also caused a reduction in serum cholesterol level without a change in the levels of serum triglycerides and phospholipids. Deficiency of both magnesium and sulfate salts in thiamine-supplemented groups decreased body weight gain and liver thiamine content with a significant elevation of serum triglycerides.