Method with high-throughput screening potential for antioxidative substances using Escherichia coli biosensor katG'::lux

J Microbiol Methods. 2015 Nov:118:78-80. doi: 10.1016/j.mimet.2015.08.018. Epub 2015 Aug 29.

Abstract

A new method is described for the rapid real-time screening of antioxidative properties using a recombinant Escherichia coli DPD2511 biosensor. This microplate technique, without time-consuming pre-incubations and handling, has potential for a high-throughput search of bioactive compounds. Special emphasis was given to obtaining highly reliable and repeatable results.

Keywords: Antioxidative activity; Bacterial biosensor; Bioscreening; Microplate technique.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / analysis*
  • Biosensing Techniques / methods*
  • Catalase / genetics
  • Catalase / metabolism
  • Escherichia coli / drug effects*
  • Escherichia coli / genetics
  • Escherichia coli Proteins / genetics
  • Escherichia coli Proteins / metabolism
  • High-Throughput Screening Assays / methods*
  • Luciferases / genetics
  • Luciferases / metabolism
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Reproducibility of Results

Substances

  • Antioxidants
  • Escherichia coli Proteins
  • Recombinant Fusion Proteins
  • Catalase
  • katG protein, E coli
  • Luciferases