Abstract
A new method is described for the rapid real-time screening of antioxidative properties using a recombinant Escherichia coli DPD2511 biosensor. This microplate technique, without time-consuming pre-incubations and handling, has potential for a high-throughput search of bioactive compounds. Special emphasis was given to obtaining highly reliable and repeatable results.
Keywords:
Antioxidative activity; Bacterial biosensor; Bioscreening; Microplate technique.
Copyright © 2015 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antioxidants / analysis*
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Biosensing Techniques / methods*
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Catalase / genetics
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Catalase / metabolism
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Escherichia coli / drug effects*
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Escherichia coli / genetics
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / metabolism
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High-Throughput Screening Assays / methods*
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Luciferases / genetics
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Luciferases / metabolism
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Reproducibility of Results
Substances
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Antioxidants
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Escherichia coli Proteins
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Recombinant Fusion Proteins
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Catalase
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katG protein, E coli
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Luciferases