Effects of sodium nitroprusside (SNP) pretreatment on UV-B stress tolerance in lettuce (Lactuca sativa L.) seedlings

Environ Sci Pollut Res Int. 2016 Jan;23(1):589-97. doi: 10.1007/s11356-015-5301-1. Epub 2015 Sep 2.

Abstract

Ultraviolet-B (UV-B) radiation is one of the most important abiotic stress factors that could influence plant growth, development, and productivity. Nitric oxide (NO) is an important plant growth regulator involved in a wide variety of physiological processes. In the present study, the possibility of enhancing UV-B stress tolerance of lettuce seedlings by the exogenous application of sodium nitroprusside (SNP) was investigated. UV-B radiation increased the activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), peroxidase (POD) and total phenolic concentrations, antioxidant capacity, and expression of phenylalanine ammonia lyase (PAL) gene in seedlings, but the combination of SNP pretreatment and UV-B enhanced antioxidant enzyme activities, total phenolic concentrations, antioxidant capacity, and PAL gene expression even more. Moreover, UV-B radiation significantly inhibited chlorophylls, carotenoid, gibberellic acid (GA), and indole-3-acetic acid (IAA) contents and increased the contents of abscisic acid (ABA), salicylic acid (SA), malondialdehyde (MDA), hydrogen peroxide (H2O2), and superoxide radical (O2•(-)) in lettuce seedlings. When SNP pretreatment was combined with the UV-B radiation, we observed alleviated chlorophylls, carotenoid, GA, and IAA inhibition and decreased content of ABA, SA, MDA, H2O2, and O2•(-) in comparison to non-pretreated stressed seedlings.

Keywords: Antioxidant enzyme; Lettuce; Phenylalanine ammonia lyase; Sodium nitroprusside; Ultraviolet-B.

MeSH terms

  • Abscisic Acid / metabolism
  • Antioxidants / metabolism
  • Ascorbate Peroxidases / metabolism
  • Catalase / metabolism
  • Chlorophyll / metabolism
  • Hydrogen Peroxide / metabolism
  • Indoleacetic Acids / metabolism
  • Lettuce / drug effects*
  • Lettuce / genetics
  • Lettuce / physiology
  • Lettuce / radiation effects*
  • Malondialdehyde / metabolism
  • Nitroprusside / pharmacology*
  • Peroxidase / metabolism
  • Plant Proteins / metabolism
  • Seedlings / drug effects
  • Seedlings / genetics
  • Seedlings / physiology*
  • Seedlings / radiation effects
  • Stress, Physiological
  • Superoxide Dismutase / metabolism

Substances

  • Antioxidants
  • Indoleacetic Acids
  • Plant Proteins
  • Chlorophyll
  • Nitroprusside
  • Malondialdehyde
  • indoleacetic acid
  • Abscisic Acid
  • Hydrogen Peroxide
  • Ascorbate Peroxidases
  • Catalase
  • Peroxidase
  • Superoxide Dismutase