Human liver-resident CD56(bright)/CD16(neg) NK cells are retained within hepatic sinusoids via the engagement of CCR5 and CXCR6 pathways

J Autoimmun. 2016 Jan:66:40-50. doi: 10.1016/j.jaut.2015.08.011. Epub 2015 Aug 30.


Rationale: The liver-specific natural killer (NK) cell population is critical for local innate immune responses, but the mechanisms that lead to their selective homing and the definition of their functionally relevance remain enigmatic.

Objectives: We took advantage of the availability of healthy human liver to rigorously define the mechanisms regulating the homing of NK cells to liver and the repertoire of receptors that distinguish liver-resident NK (lr-NK) cells from circulating counterparts.

Findings: Nearly 50% of the entire liver NK cell population is composed of functionally relevant CD56(bright) lr-NK cells that localize within hepatic sinusoids. CD56(bright) lr-NK cells express CD69, CCR5 and CXCR6 and this unique repertoire of chemokine receptors is functionally critical as it determines selective migration in response to the chemotactic stimuli exerted by CCL3, CCL5 and CXCL16. Here, we also show that hepatic sinusoids express CCL3(pos) Kupffer cells, CXCL16(pos) endothelial cells and CCL5(pos) T and NK lymphocytes. The selective presence of these chemokines in sinusoidal spaces creates a unique tissue niche for lr-CD56(bright) NK cells that constitutively express CCR5 and CXCR6. CD56(bright) lr-NK cells co-exist with CD56(dim) conventional NK (c-NK) cells that are, interestingly, transcriptionally and phenotypically similar to their peripheral circulating counterparts. Indeed, CD56(dim) c-NK cells lack expression of CD69, CCR5, and CXCR6 but express selectins, integrins and CX3CR1.

Conclusion: Our findings disclosing the phenotypic and functional differences between lr-Nk cells and c-NK cells are critical to distinguish liver-specific innate immune responses. Hence, any therapeutic attempts at modifying the large population of CD56(bright) lr-NK cells will require modification of hepatic CCR5 and CXCR6.

Keywords: Cherokees Chemokine receptors; Homing of hepatic NK cells; Liver immunology.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antigens, CD / immunology
  • Antigens, CD / metabolism
  • Antigens, Differentiation, T-Lymphocyte / immunology
  • Antigens, Differentiation, T-Lymphocyte / metabolism
  • CD56 Antigen / immunology
  • CD56 Antigen / metabolism
  • Cell Movement
  • Endothelial Cells / metabolism
  • Humans
  • Killer Cells, Natural / immunology*
  • Killer Cells, Natural / metabolism
  • Kupffer Cells / immunology
  • Lectins, C-Type / immunology
  • Lectins, C-Type / metabolism
  • Liver / blood supply
  • Liver / cytology*
  • Liver / immunology*
  • Liver / metabolism
  • Receptors, CCR5 / metabolism*
  • Receptors, CXCR6
  • Receptors, Chemokine / metabolism*
  • Receptors, IgG / immunology
  • Receptors, IgG / metabolism
  • Receptors, Virus / metabolism*
  • Signal Transduction / immunology
  • T-Lymphocytes / immunology


  • Antigens, CD
  • Antigens, Differentiation, T-Lymphocyte
  • CCR5 protein, human
  • CD56 Antigen
  • CD69 antigen
  • CXCR6 protein, human
  • Lectins, C-Type
  • Receptors, CCR5
  • Receptors, CXCR6
  • Receptors, Chemokine
  • Receptors, IgG
  • Receptors, Virus