Discovery of a Selective and CNS Penetrant Negative Allosteric Modulator of Metabotropic Glutamate Receptor Subtype 3 with Antidepressant and Anxiolytic Activity in Rodents

J Med Chem. 2015 Sep 24;58(18):7485-500. doi: 10.1021/acs.jmedchem.5b01005. Epub 2015 Sep 10.

Abstract

Previous preclinical work has demonstrated the therapeutic potential of antagonists of the group II metabotropic glutamate receptors (mGlus). Still, compounds that are selective for the individual group II mGlus (mGlu2 and mGlu3) have been scarce. There remains a need for such compounds with the balance of properties suitable for convenient use in a wide array of rodent behavioral studies. We describe here the discovery of a selective mGlu3 NAM 106 (VU0650786) suitable for in vivo work. Compound 106 is a member of a series of 5-aryl-6,7-dihydropyrazolo[1,5-a]pyrazine-4(5H)-one compounds originally identified as a mGlu5 positive allosteric modulator (PAM) chemotype. Its suitability for use in rodent behavioral models has been established by extensive in vivo PK studies, and the behavioral experiments presented here with compound 106 represent the first examples in which an mGlu3 NAM has demonstrated efficacy in models where prior efficacy had previously been noted with nonselective group II antagonists.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Allosteric Regulation
  • Animals
  • Anti-Anxiety Agents / chemistry*
  • Anti-Anxiety Agents / pharmacokinetics
  • Anti-Anxiety Agents / pharmacology
  • Antidepressive Agents / chemistry*
  • Antidepressive Agents / pharmacokinetics
  • Antidepressive Agents / pharmacology
  • Brain / metabolism*
  • Calcium / metabolism
  • Dogs
  • Heterocyclic Compounds, 2-Ring / chemistry*
  • Heterocyclic Compounds, 2-Ring / pharmacokinetics
  • Heterocyclic Compounds, 2-Ring / pharmacology
  • Humans
  • Madin Darby Canine Kidney Cells
  • Mice
  • Microsomes, Liver / metabolism
  • Permeability
  • Pyridines / chemistry*
  • Pyridines / pharmacokinetics
  • Pyridines / pharmacology
  • Rats
  • Receptors, Metabotropic Glutamate / metabolism*
  • Stereoisomerism
  • Structure-Activity Relationship

Substances

  • Anti-Anxiety Agents
  • Antidepressive Agents
  • Heterocyclic Compounds, 2-Ring
  • Pyridines
  • Receptors, Metabotropic Glutamate
  • VU0650786
  • metabotropic glutamate receptor 3
  • Calcium