Growth and Quantification of MERS-CoV Infection

doi:10.1002/9780471729259.mc15e02s37

. 2015 May 1;37(1):15E.2.1-9.

doi: 10.1002/9780471729259.mc15e02s37.

Growth and Quantification of MERS-CoV Infection

Christopher M Coleman¹, Matthew B Frieman¹

Affiliations

PMID: 26344219
PMCID: PMC4735735
DOI: 10.1002/9780471729259.mc15e02s37

Growth and Quantification of MERS-CoV Infection

Christopher M Coleman et al. Curr Protoc Microbiol. 2015.

. 2015 May 1;37(1):15E.2.1-9.

doi: 10.1002/9780471729259.mc15e02s37.

Authors

Christopher M Coleman¹, Matthew B Frieman¹

Affiliation

¹ University of Maryland School of Medicine, Microbiology and Immunology, Baltimore, Maryland.

PMID: 26344219
PMCID: PMC4735735
DOI: 10.1002/9780471729259.mc15e02s37

Abstract

Middle East respiratory syndrome coronavirus (MERS-CoV) is an emerging highly pathogenic respiratory virus. Although MERS-CoV only emerged in 2012, we and others have developed assays to grow and quantify infectious MERS-CoV and RNA products of replication in vitro. MERS-CoV is able to infect a range of cell types, but replicates to high titers in Vero E6 cells. Protocols for the propagation and quantification of MERS-CoV are presented.

Keywords: MERS; coronavirus; infection; plaque assay; quantification; virology.

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References

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1. Coleman, C.M. , Matthews, K.L. , Goicochea, L. , and Frieman, M.B. , 2013. Wild type and innate immune deficient mice are not susceptible to the Middle East Respiratory Syndrome Coronavirus. J. Gen. Virol. 95(Pt 2):408‐412. - PMC - PubMed
1. de Wit, E. , Prescott, J. , Baseler, L. , Bushmaker, T. , Thomas, T. , Lackemeyer, M.G. , Martellaro, C. , Milne‐Price, S. , Haddock, E. , Haagmans, B.L. , Feldmann, H. , and Munster, V.J. , 2013a. The Middle East respiratory syndrome coronavirus (MERS‐CoV) does not replicate in Syrian hamsters. PloS One 8:e69127. - PMC - PubMed
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1. Falzarano, D. , de Wit, E. , Feldmann, F. , Rasmussen, A.L. , Okumura, A. , Peng, X. , Thomas, M.J. , van Doremalen, N. , Haddock, E. , Nagy, L. , LaCasse, R. , Liu, T. , Zhu, J. , McLellan, J.S. , Scott, D.P. , Katze, M.G. , Feldmann, H. , and Munster, V.J. , 2014. Infection with MERS‐CoV causes lethal pneumonia in the common marmoset. PLoS Pathog. 10:e1004250. - PMC - PubMed

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[1] Chan, J.F. , Chan, K.H. , Choi, G.K. , To, K.K. , Tse, H. , Cai, J.P. , Yeung, M.L. , Cheng, V.C. , Chen, H. , Che, X.Y. , Lau, S.K. , Woo, P.C. , and Yuen, K.Y. , 2013. Differential cell line susceptibility to the emerging novel human betacoronavirus 2c EMC/2012: Implications for disease pathogenesis and clinical manifestation. J. Infect. Dis. 207:1743‐1752. - PMC - PubMed

[2] Chan, J.F. , Chan, K.H. , Choi, G.K. , To, K.K. , Tse, H. , Cai, J.P. , Yeung, M.L. , Cheng, V.C. , Chen, H. , Che, X.Y. , Lau, S.K. , Woo, P.C. , and Yuen, K.Y. , 2013. Differential cell line susceptibility to the emerging novel human betacoronavirus 2c EMC/2012: Implications for disease pathogenesis and clinical manifestation. J. Infect. Dis. 207:1743‐1752. - PMC - PubMed

[3] Coleman, C.M. , Matthews, K.L. , Goicochea, L. , and Frieman, M.B. , 2013. Wild type and innate immune deficient mice are not susceptible to the Middle East Respiratory Syndrome Coronavirus. J. Gen. Virol. 95(Pt 2):408‐412. - PMC - PubMed

[4] Coleman, C.M. , Matthews, K.L. , Goicochea, L. , and Frieman, M.B. , 2013. Wild type and innate immune deficient mice are not susceptible to the Middle East Respiratory Syndrome Coronavirus. J. Gen. Virol. 95(Pt 2):408‐412. - PMC - PubMed

[5] de Wit, E. , Prescott, J. , Baseler, L. , Bushmaker, T. , Thomas, T. , Lackemeyer, M.G. , Martellaro, C. , Milne‐Price, S. , Haddock, E. , Haagmans, B.L. , Feldmann, H. , and Munster, V.J. , 2013a. The Middle East respiratory syndrome coronavirus (MERS‐CoV) does not replicate in Syrian hamsters. PloS One 8:e69127. - PMC - PubMed

[6] de Wit, E. , Prescott, J. , Baseler, L. , Bushmaker, T. , Thomas, T. , Lackemeyer, M.G. , Martellaro, C. , Milne‐Price, S. , Haddock, E. , Haagmans, B.L. , Feldmann, H. , and Munster, V.J. , 2013a. The Middle East respiratory syndrome coronavirus (MERS‐CoV) does not replicate in Syrian hamsters. PloS One 8:e69127. - PMC - PubMed

[7] de Wit, E. , Rasmussen, A.L. , Falzarano, D. , Bushmaker, T. , Feldmann, F. , Brining, D.L. , Fischer, E.R. , Martellaro, C. , Okumura, A. , Chang, J. , Scott, D. , Benecke, A.G. , Katze, M.G. , Feldmann, H. , and Munster, V.J. , 2013b. Middle East respiratory syndrome coronavirus (MERS‐CoV) causes transient lower respiratory tract infection in rhesus macaques. Proc. Natl. Acad. Sci. U.S.A. 110:16598‐16603. - PMC - PubMed

[8] de Wit, E. , Rasmussen, A.L. , Falzarano, D. , Bushmaker, T. , Feldmann, F. , Brining, D.L. , Fischer, E.R. , Martellaro, C. , Okumura, A. , Chang, J. , Scott, D. , Benecke, A.G. , Katze, M.G. , Feldmann, H. , and Munster, V.J. , 2013b. Middle East respiratory syndrome coronavirus (MERS‐CoV) causes transient lower respiratory tract infection in rhesus macaques. Proc. Natl. Acad. Sci. U.S.A. 110:16598‐16603. - PMC - PubMed

[9] Falzarano, D. , de Wit, E. , Feldmann, F. , Rasmussen, A.L. , Okumura, A. , Peng, X. , Thomas, M.J. , van Doremalen, N. , Haddock, E. , Nagy, L. , LaCasse, R. , Liu, T. , Zhu, J. , McLellan, J.S. , Scott, D.P. , Katze, M.G. , Feldmann, H. , and Munster, V.J. , 2014. Infection with MERS‐CoV causes lethal pneumonia in the common marmoset. PLoS Pathog. 10:e1004250. - PMC - PubMed

[10] Falzarano, D. , de Wit, E. , Feldmann, F. , Rasmussen, A.L. , Okumura, A. , Peng, X. , Thomas, M.J. , van Doremalen, N. , Haddock, E. , Nagy, L. , LaCasse, R. , Liu, T. , Zhu, J. , McLellan, J.S. , Scott, D.P. , Katze, M.G. , Feldmann, H. , and Munster, V.J. , 2014. Infection with MERS‐CoV causes lethal pneumonia in the common marmoset. PLoS Pathog. 10:e1004250. - PMC - PubMed

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Growth and Quantification of MERS-CoV Infection

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Growth and Quantification of MERS-CoV Infection

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