Clipping of Telomere from Human Chromosomes Using a Chemistry-Based Artificial Restriction DNA Cutter

Curr Protoc Nucleic Acid Chem. 2015 Jun 2;61:6.13.1-6.13.13. doi: 10.1002/0471142700.nc0613s61.

Abstract

The detection of individual telomere lengths of human chromosomes can provide crucial information on genome stability, cancer, and telomere-related diseases. However, current methods to measure telomere length entail shortcomings that have limited their use. Recently, we have developed a method for detection of individual telomere lengths (DITL) that uses a chemistry-based DNA-cutting approach. The most beneficial feature of the DITL approach is to cleave the sequence adjacent to the telomere followed by resolution of the telomere length at the nucleotide level of a single chromosome. In this unit, a protocol for successful detection of individual telomere lengths from individual chromosomes is described in detail.

Keywords: DNA cleavage; biotechnology; peptide nucleic acid; telomere length.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biotechnology / methods
  • Chromosomes, Human / genetics
  • Humans
  • Peptide Nucleic Acids / chemistry*
  • Telomere / genetics

Substances

  • Peptide Nucleic Acids