Clipping of Telomere from Human Chromosomes Using a Chemistry-Based Artificial Restriction DNA Cutter

Takumi Ishizuka; Yan Xu; Makoto Komiyama

doi:10.1002/0471142700.nc0613s61

Clipping of Telomere from Human Chromosomes Using a Chemistry-Based Artificial Restriction DNA Cutter

Curr Protoc Nucleic Acid Chem. 2015 Jun 2:61:6.13.1-6.13.13. doi: 10.1002/0471142700.nc0613s61.

Authors

Takumi Ishizuka¹, Yan Xu¹, Makoto Komiyama²

Affiliations

¹ Division of Chemistry, Department of Medical Sciences, University of Miyazaki, Miyazaki, Japan.
² Life Science Center of Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, Tsukuba, Japan.

PMID: 26344230
DOI: 10.1002/0471142700.nc0613s61

Abstract

The detection of individual telomere lengths of human chromosomes can provide crucial information on genome stability, cancer, and telomere-related diseases. However, current methods to measure telomere length entail shortcomings that have limited their use. Recently, we have developed a method for detection of individual telomere lengths (DITL) that uses a chemistry-based DNA-cutting approach. The most beneficial feature of the DITL approach is to cleave the sequence adjacent to the telomere followed by resolution of the telomere length at the nucleotide level of a single chromosome. In this unit, a protocol for successful detection of individual telomere lengths from individual chromosomes is described in detail.

Keywords: DNA cleavage; biotechnology; peptide nucleic acid; telomere length.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biotechnology / methods
Chromosomes, Human / genetics
Humans
Peptide Nucleic Acids / chemistry*
Telomere / genetics

Substances

Peptide Nucleic Acids