In vivo capture and label-free detection of early metastatic cells

Nat Commun. 2015 Sep 8;6:8094. doi: 10.1038/ncomms9094.

Abstract

Breast cancer is a leading cause of death for women, with mortality resulting from metastasis. Metastases are often detected once tumour cells affect the function of solid organs, with a high disease burden limiting effective treatment. Here we report a method for the early detection of metastasis using an implanted scaffold to recruit and capture metastatic cells in vivo, which achieves high cell densities and reduces the tumour burden within solid organs 10-fold. Recruitment is associated with infiltration of immune cells, which include Gr1(hi)CD11b(+) cells. We identify metastatic cells in the scaffold through a label-free detection system using inverse spectroscopic optical coherence tomography, which identifies changes to nanoscale tissue architecture associated with the presence of tumour cells. For patients at risk of recurrence, scaffold implantation following completion of primary therapy has the potential to identify metastatic disease at the earliest stage, enabling initiation of therapy while the disease burden is low.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adenocarcinoma / diagnosis*
  • Adenocarcinoma / secondary
  • Animals
  • Biocompatible Materials*
  • Breast Neoplasms / diagnosis*
  • Breast Neoplasms / pathology
  • Disease Models, Animal
  • Early Detection of Cancer
  • Female
  • Humans
  • Liver Neoplasms / diagnosis*
  • Liver Neoplasms / secondary
  • Lung Neoplasms / diagnosis*
  • Lung Neoplasms / secondary
  • Mice
  • Neoplasm Metastasis
  • Neoplasm Transplantation
  • Neoplastic Cells, Circulating*
  • Prostheses and Implants
  • Tissue Scaffolds*
  • Tomography, Optical Coherence
  • Tumor Burden

Substances

  • Biocompatible Materials