[Increased manganese superoxide dismutase and cyclin B1 expression in carnosine-induced inhibition of glioblastoma cell proliferation]

Biomed Khim. Jul-Aug 2015;61(4):510-8. doi: 10.18097/PBMC20156104510.
[Article in Russian]

Abstract

Carnosine is an endogenous dipeptide with antiproliferative properties. Here we show that carnosine selectively inhibits proliferation of human glioblastoma cells (U-118-MG) compared to breast (MB231) and oral (Cal27 and FaDu) cancer cells. Carnosine-induced inhibition of U-118-MG proliferation is associated with a significant: decrease in cellular reactive oxygen species levels, increase in manganese superoxide dismutase (MnSOD) and increase in cyclin B1 expression resulting in G2-block. We conclude that the antiproliferative property of carnosine is due to its ability to enhance MnSOD and cyclin B1 expression. These results will be of significance to the potential application of carnosine in brain cancer therapy.

Karnozin predstavliaet soboĭ éndogennyĭ dipeptid, obladaiushchiĭ antiproliferativnymi svoĭstvami. V dannoĭ rabote my pokazali, chto karnozin izbiratel'no ingibiruet rost kletok glioblastomy cheloveka (U-118-MG) po sravneniiu s kletkami kartsinomy gorla (FaDu), rta (Cal27) i molochnoĭ zhelezy (MB231). Zamedlenie proliferatsii kletok U-118-MG soprovozhdalos' znachitel'nym snizheniem vnutrikletochnogo urovnia aktivnykh form kisloroda, uvelicheniem ékspressii margantsevoĭ superoksiddismutazy (MnSOD) i tsiklina V1, chto privodilo k formirovaniiu G2-bloka. Na osnove poluchennykh dannykh, bylo sdelano zakliuchenie o tom, chto antiproliferativnye svoĭstva karnozina obuslovleny ego sposobnost'iu usilivat' ékspressiiu MnSOD i tsiklina V1. Poluchennye rezul'taty mogut byt' ispol'zovany dlia razrabotki metodov primeneniia karnozina v terapii opukholevykh zabolevaniĭ golovnogo mozga.

Keywords: G2-block; MnSOD; anserine; carnosine; cell cycle; cyclin B1.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / pharmacology*
  • Carnosine / pharmacology*
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Cyclin B1 / agonists
  • Cyclin B1 / genetics*
  • Cyclin B1 / metabolism
  • Dose-Response Relationship, Drug
  • G2 Phase Cell Cycle Checkpoints / drug effects
  • Gene Expression Regulation, Neoplastic / drug effects
  • Humans
  • Neuroglia / drug effects*
  • Neuroglia / metabolism
  • Neuroglia / pathology
  • Organ Specificity
  • Reactive Oxygen Species / antagonists & inhibitors*
  • Reactive Oxygen Species / metabolism
  • Superoxide Dismutase / genetics*
  • Superoxide Dismutase / metabolism

Substances

  • Antineoplastic Agents
  • Cyclin B1
  • Reactive Oxygen Species
  • Carnosine
  • Superoxide Dismutase