Complete Genome Sequence of a High Lipid-Producing Strain of Mucor circinelloides WJ11 and Comparative Genome Analysis with a Low Lipid-Producing Strain CBS 277.49

Xin Tang; Lina Zhao; Haiqin Chen; Yong Q Chen; Wei Chen; Yuanda Song; Colin Ratledge

doi:10.1371/journal.pone.0137543

Complete Genome Sequence of a High Lipid-Producing Strain of Mucor circinelloides WJ11 and Comparative Genome Analysis with a Low Lipid-Producing Strain CBS 277.49

PLoS One. 2015 Sep 9;10(9):e0137543. doi: 10.1371/journal.pone.0137543. eCollection 2015.

Authors

Xin Tang¹, Lina Zhao¹, Haiqin Chen², Yong Q Chen², Wei Chen², Yuanda Song¹, Colin Ratledge³

Affiliations

¹ State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, P.R. China.
² State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, P.R. China; Synergistic Innovation Center for Food Safety and Nutrition, Wuxi, P.R. China.
³ Department of Biological Sciences, University of Hull, Hull, United Kingdom.

Abstract

The genome of a high lipid-producing fungus Mucor circinelloides WJ11 (36% w/w lipid, cell dry weight, CDW) was sequenced and compared with that of the low lipid-producing strain, CBS 277.49 (15% w/w lipid, CDW), which had been sequenced by Joint Genome Institute. The WJ11 genome assembly size was 35.4 Mb with a G+C content of 39.7%. The general features of WJ11 and CBS 277.49 indicated that they have close similarity at the level of gene order and gene identity. Whole genome alignments with MAUVE revealed the presence of numerous blocks of homologous regions and MUMmer analysis showed that the genomes of these two strains were mostly co-linear. The central carbon and lipid metabolism pathways of these two strains were reconstructed and the numbers of genes encoding the enzymes related to lipid accumulation were compared. Many unique genes coding for proteins involved in cell growth, carbohydrate metabolism and lipid metabolism were identified for each strain. In conclusion, our study on the genome sequence of WJ11 and the comparative genomic analysis between WJ11 and CBS 277.49 elucidated the general features of the genome and the potential mechanism of high lipid accumulation in strain WJ11 at the genomic level. The different numbers of genes and unique genes involved in lipid accumulation may play a role in the high oleaginicity of strain WJ11.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosome Mapping
Genome, Fungal*
Genomics
Lipid Metabolism / genetics*
Mucor / genetics*
Sequence Analysis, DNA

Grants and funding

This work was supported by the National Natural Science Foundation of China (31271812, 81071685, 21276108), the National High Technology Research and Development Program of China (2012AA022105C), the Program for Changjiang Scholars and Innovative Research Team in University (IRT1249), the Program for New Century Excellent Talents (NCET-13-0831), and the Strategic Merieux Research Grant. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.