Synthesis and Evaluation of DNA Based Quantum Dot Fluorescence In Situ Hybridization (FISH) Probe for Telomere Detection

J Nanosci Nanotechnol. 2015 Feb;15(2):1708-13. doi: 10.1166/jnn.2015.9500.


Efficient oligonucleotide probe design and synthesis based on polymer-coated CdSe/ZnS quantum dot (QD) is demonstrated for detection of telomeres in human monocyte and Leishmania major, a protozoan pathogenic parasite. The highly photoluminescent polymer-coated QDs conjugated with various length of telomere probe sequences were prepared via carbodiimide chemistry and characterized. Specific detection of telomere was observed when DNA sequence was (CCCAAT)n (n = 5 or 3) probe sequence, rather than (GGGTTA)n (n = 3, 5, 8). The sensitivity and specificity were comparable with commercially available PNA probe for human telomere detection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA / chemistry
  • DNA / genetics*
  • DNA Probes / genetics
  • Equipment Design
  • Equipment Failure Analysis
  • In Situ Hybridization, Fluorescence / methods*
  • Leishmania major / genetics*
  • Materials Testing
  • Molecular Sequence Data
  • Nanoconjugates / chemistry
  • Quantum Dots*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Sequence Analysis, DNA / methods*
  • Telomere / genetics*


  • DNA Probes
  • Nanoconjugates
  • DNA