Discovering New Biology through Sequencing of RNA

Plant Physiol. 2015 Nov;169(3):1524-31. doi: 10.1104/pp.15.01081. Epub 2015 Sep 9.

Abstract

Sequencing of RNA (RNA-Seq) was invented approximately 1 decade ago and has since revolutionized biological research. This update provides a brief historic perspective on the development of RNA-Seq and then focuses on the application of RNA-Seq in qualitative and quantitative analyses of transcriptomes. Particular emphasis is given to aspects of data analysis. Since the wet-lab and data analysis aspects of RNA-Seq are still rapidly evolving and novel applications are continuously reported, a printed review will be rapidly outdated and can only serve to provide some examples and general guidelines for planning and conducting RNA-Seq studies. Hence, selected references to frequently update online resources are given.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Gene Expression Regulation, Plant
  • High-Throughput Nucleotide Sequencing / methods
  • Plants / genetics
  • Plants / metabolism*
  • RNA, Plant / genetics*
  • Sequence Analysis, RNA / methods*
  • Transcription, Genetic

Substances

  • RNA, Plant