MiRNA-101 inhibits breast cancer growth and metastasis by targeting CX chemokine receptor 7

Oncotarget. 2015 Oct 13;6(31):30818-30. doi: 10.18632/oncotarget.5067.


Whereas miR-101 is involved in the development and progression of breast cancer, the underlying molecular mechanisms remain to be elucidated. Here, we report that miR-101 expression is inversely correlated with the clinical stage, lymph node metastasis and prognosis in breast cancers. Introduction of miR-101 inhibited breast cancer cell proliferation and invasion in vitro and suppressed tumor growth and lung metastasis of in vivo. CX chemokine receptor 7 (CXCR7) is a direct target of miR-101, positively correlating with the histological grade and the incidence of lymph node metastasis in breast cancer patients. The effects of miR-101 were mimicked and counteracted by CXCR7 depletion and overexpression, respectively. STAT3 signaling downstream of CXCR7 is involved in miR-101 regulation of breast cancer cell behaviors. These findings have implications for the potential application of miR-101 in breast cancer treatment.

Keywords: CXCR7; breast cancer; metastasis; miR-101; proliferation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis
  • Breast Neoplasms / genetics
  • Breast Neoplasms / metabolism*
  • Breast Neoplasms / mortality
  • Breast Neoplasms / pathology
  • Breast Neoplasms / surgery
  • Cell Line, Tumor
  • Cell Movement*
  • Cell Proliferation*
  • Female
  • Gene Expression Regulation, Neoplastic
  • HEK293 Cells
  • Humans
  • Kaplan-Meier Estimate
  • Lung Neoplasms / genetics
  • Lung Neoplasms / metabolism*
  • Lung Neoplasms / mortality
  • Lung Neoplasms / prevention & control
  • Lung Neoplasms / secondary
  • Lymphatic Metastasis
  • Mice, Inbred BALB C
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Neoplasm Grading
  • Neoplasm Invasiveness
  • RNA Interference
  • Receptors, CXCR / genetics
  • Receptors, CXCR / metabolism*
  • STAT3 Transcription Factor / metabolism
  • Signal Transduction
  • Time Factors
  • Transfection
  • Tumor Burden


  • ACKR3 protein, human
  • MIRN101 microRNA, human
  • MicroRNAs
  • Receptors, CXCR
  • STAT3 Transcription Factor
  • STAT3 protein, human