Integrated, multi-scale, spatial-temporal cell biology--A next step in the post genomic era

Methods. 2016 Mar 1:96:3-5. doi: 10.1016/j.ymeth.2015.09.007. Epub 2015 Sep 7.


New microscopic approaches, high-throughput imaging, and gene editing promise major new insights into cellular behaviors. When coupled with genomic and other 'omic information and "mined" for correlations and associations, a new breed of powerful and useful cellular models should emerge. These top down, coarse-grained, and statistical models, in turn, can be used to form hypotheses merging with fine-grained, bottom up mechanistic studies and models that are the back bone of cell biology. The goal of the Allen Institute for Cell Science is to develop the top down approach by developing a high throughput microscopy pipeline that is integrated with modeling, using gene edited hiPS cell lines in various physiological and pathological contexts. The output of these experiments and models will be an "animated" cell, capable of integrating and analyzing image data generated from experiments and models.

Keywords: Cell Science; Cellular behaviors; Postgenomic era; Spatio-temporal cell biology.

Publication types

  • Review

MeSH terms

  • Cell Biology / trends*
  • Genomics / instrumentation
  • Genomics / methods
  • High-Throughput Screening Assays
  • Humans
  • Induced Pluripotent Stem Cells / cytology
  • Induced Pluripotent Stem Cells / metabolism
  • Microscopy / instrumentation
  • Microscopy / methods*
  • Molecular Imaging / instrumentation
  • Molecular Imaging / methods*
  • Single-Cell Analysis / instrumentation
  • Single-Cell Analysis / methods*