Accelerated reproductive aging in females lacking a novel centromere protein SYCP2L

Hum Mol Genet. 2015 Nov 15;24(22):6505-14. doi: 10.1093/hmg/ddv359. Epub 2015 Sep 11.


Menopause results from loss of ovarian function and marks the end of a woman's reproductive life. Alleles of the human SYCP2L locus are associated with age at natural menopause (ANM). SYCP2L is a paralogue of the synaptonemal complex protein SYCP2 and is expressed exclusively in oocytes. Here we report that SYCP2L localizes to centromeres of dictyate stage oocytes, which represent the limited pool of primordial oocytes that are formed perinatally and remain arrested till ovulation. Centromere localization of SYCP2L requires its C-terminal portion, which is missing in truncated variants resulting from low-frequency nonsense mutations identified in humans. Female mice lacking SYCP2L undergo a significantly higher progressive loss of oocytes with age compared with wild-type females and are less fertile. Specifically, the pool of primordial oocytes becomes more rapidly depleted in SYCP2L-deficient than in wild-type females, such that with aging, fewer oocytes undergo maturation in developing follicles. We find that a human SYCP2L intronic single nucleotide polymorphism (SNP) rs2153157, which is associated with ANM, changes the splicing efficiency of U12-type minor introns and may therefore regulate the steady-state amount of SYCP2L transcript. Furthermore, the more efficiently spliced allele of this intronic SNP in SYCP2L is associated with increased ANM. Our results suggest that SYCP2L promotes the survival of primordial oocytes and thus provide functional evidence for its association with ANM in humans.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Aging / genetics
  • Alleles
  • Animals
  • Cell Culture Techniques
  • Cell Cycle Proteins
  • Centromere / metabolism
  • Chromosomal Proteins, Non-Histone / deficiency
  • Chromosomal Proteins, Non-Histone / genetics
  • DNA-Binding Proteins / deficiency*
  • DNA-Binding Proteins / genetics
  • Female
  • Fertility / genetics
  • Humans
  • Menopause / genetics
  • Menopause / metabolism
  • Menopause / physiology*
  • Mice
  • Oocytes / metabolism*
  • Ovarian Follicle / metabolism
  • Ovary / cytology
  • Ovary / metabolism
  • Ovary / physiology
  • Ovulation / physiology
  • Polymorphism, Single Nucleotide
  • Reproduction / genetics


  • Cell Cycle Proteins
  • Chromosomal Proteins, Non-Histone
  • DNA-Binding Proteins
  • SYCP2 protein, human
  • Sycp2 protein, mouse