Psychrobacter fjordensis sp. nov., a psychrotolerant bacterium isolated from an Arctic fjord in Svalbard

Antonie Van Leeuwenhoek. 2015 Dec;108(6):1283-1292. doi: 10.1007/s10482-015-0580-6. Epub 2015 Sep 11.

Abstract

A Gram-negative, non-motile, non-spore-forming, psychrotolerant and halotolerant bacterium designated BSw21516B(T), was obtained from seawater in Kongsfjorden, a glacial fjord in the Arctic Svalbard and subjected to taxonomic analysis using a polyphasic approach. This bacterium was observed to optimally grow at 25-29 °C; between at 4 and 34 °C, but not at >35 °C; and in the presence of 0-8 % (w/v) NaCl at an optimum concentration of 2-5 % (w/v) NaCl. Strain BSw21516B(T) was found to contain Ubiquinone-8 (Q-8) as a predominant respiratory lipoquinone and C18:1 ω9c and summed feature 3 (C16:1 ω7c and/or iso-C15:0 2-OH) as predominant cellular fatty acids. Phylogenetic analysis of 16S rRNA and gyrB gene sequences showed that this isolate belongs to the genus Psychrobacter and is closely related to Psychrobacter fozii LMG 21280(T), which was isolated from a sediment sample in Antarctica. DNA hybridization experiments revealed a low level of DNA-DNA relatedness (less than 58.6 %) between strain BSw21516B(T) and its closest relatives. Based on these results a new species Psychrobacter fjordensis sp. nov. is proposed (type strain BSw21516B(T) = KCTC 42279(T) = CCTCC AB 2014020(T)).

Keywords: Arctic; Kongsfjorden; Psychrobacter; Psychrobacter fjordensis sp. nov..

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arctic Regions
  • Bacterial Typing Techniques
  • Cluster Analysis
  • Cytosol / chemistry
  • DNA Gyrase / genetics
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Estuaries
  • Fatty Acids / analysis
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Phylogeny
  • Psychrobacter / classification*
  • Psychrobacter / genetics
  • Psychrobacter / isolation & purification*
  • Psychrobacter / physiology
  • Quinones / analysis
  • RNA, Ribosomal, 16S / genetics
  • Seawater / microbiology*
  • Sequence Analysis, DNA
  • Sodium Chloride / metabolism
  • Svalbard
  • Temperature

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • Quinones
  • RNA, Ribosomal, 16S
  • Sodium Chloride
  • DNA Gyrase