Identification of key residues in Debaryomyces hansenii carbonyl reductase for highly productive preparation of (S)-aryl halohydrins

Chem Commun (Camb). 2015 Nov 7;51(86):15728-31. doi: 10.1039/c5cc06796k. Epub 2015 Sep 14.

Abstract

Key residues of Debaryomyces hansenii carbonyl reductase in the determination of the reducing activity towards aryl haloketones were identified through combinatorial mutation of conserved residues. This study provides a green and efficient biocatalyst for the synthesis of (S)-aryl halohydrins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcohol Oxidoreductases / chemistry*
  • Alcohol Oxidoreductases / genetics
  • Catalysis
  • Chlorohydrins / chemical synthesis*
  • Fungal Proteins / chemistry*
  • Fungal Proteins / genetics
  • Models, Molecular
  • Mutation
  • Saccharomycetales / enzymology*
  • Saccharomycetales / genetics

Substances

  • Chlorohydrins
  • Fungal Proteins
  • Alcohol Oxidoreductases