Abstract
Key residues of Debaryomyces hansenii carbonyl reductase in the determination of the reducing activity towards aryl haloketones were identified through combinatorial mutation of conserved residues. This study provides a green and efficient biocatalyst for the synthesis of (S)-aryl halohydrins.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alcohol Oxidoreductases / chemistry*
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Alcohol Oxidoreductases / genetics
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Catalysis
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Chlorohydrins / chemical synthesis*
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Fungal Proteins / chemistry*
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Fungal Proteins / genetics
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Models, Molecular
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Mutation
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Saccharomycetales / enzymology*
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Saccharomycetales / genetics
Substances
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Chlorohydrins
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Fungal Proteins
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Alcohol Oxidoreductases