Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Sep 8;16(9):21575-90.
doi: 10.3390/ijms160921575.

Effects of Low Molecular Weight Yeast β-Glucan on Antioxidant and Immunological Activities in Mice

Affiliations
Free PMC article

Effects of Low Molecular Weight Yeast β-Glucan on Antioxidant and Immunological Activities in Mice

Na Lei et al. Int J Mol Sci. .
Free PMC article

Abstract

To evaluate the antioxidant and immune effects of low molecular yeast β-glucan on mice, three sulfated glucans from Saccharomyces cerevisiae (sGSCs) with different molecular weight (MW) and degrees of sulfation (DS) were prepared. The structures of the sGSCs were analyzed through high performance liquid chromatography-gel permeation chromatography (HPLC-GPC) and Fourier transform infrared spectroscopy (FTIR). sGSC1, sGSC2, and sGSC3 had MW of 12.9, 16.5 and 19.2 kDa, respectively, and DS of 0.16, 0.24 and 0.27, respectively. In vitro and in vivo experiments were conducted to evaluate the antioxidant and immunological activities of the sGSCs. In vitro experiment, the reactive oxygen species (ROS) scavenging activities were determined. In vivo experiment, 50 male BALB/c mice were divided into five groups. The sGSC1, sGSC2 and sGSC3 treatment groups received the corresponding sGSCs at 50 mg/kg/day each. The GSC (glucans from Saccharomyces cerevisiae) treatment group received 50 mg/kg/day GSC. The normal control group received equal volume of physiological saline solution. All treatments were administered intragastrically for 14 day. Results showed that sGSC1, sGSC2 and sGSC3 can scavenge 1,1-diphenyl-2-picryl-hydrazyl (DPPH), superoxide, and hydroxyl radicals in vitro. The strength of the radical scavenging effects of the sGSCs was in the order of sGSC1 > sGSC2 > sGSC3. Oral administration of sGSC1 significantly improved serum catalase (CAT) and glutathione peroxidase (GSH-Px) activities and decreased malondialdehyde (MDA) level in mice. sGSC1 significantly improved the spleen and thymus indexes and the lymphocyte proliferation, effectively enhanced the percentage of CD4⁺ T cells, decreased the percentage of CD8⁺ T cells, and elevated the CD4⁺/CD8⁺ ratio. sGSC1 significantly promoted the secretion of IL-2 and IFN-γ. These results indicate that sGSC1 with low MW and DS has better antioxidant and immunological activities than the other sGSCs, and sGSC1 could be used as a new antioxidant and immune-enhancing agent.

Keywords: Saccharomyces cerevisiae; antioxidant activity; immune activity; sulfated glucan.

Figures

Figure 1
Figure 1
FTIR of GSC (A), sGSC1 (B), sGSC2 (C) and sGSC3 (D).
Figure 2
Figure 2
Antioxidant effects of sGSCs: (A) DPPH radical scavenging activity; (B) Superoxide anion radical scavenging activity; and (C) Hydroxyl radical scavenging activity.

Similar articles

See all similar articles

Cited by 7 articles

See all "Cited by" articles

References

    1. Zhu F.M., Du B., Bian Z.X., Xu B.J. β-Glucans from edible and medicinal mushrooms: Characteristics, physicochemical and biological activities. J. Food Compos. Anal. 2015;41:165–173. doi: 10.1016/j.jfca.2015.01.019. - DOI
    1. Rieder A., Samuelsen A.B. Do cereal mixed-linked β-glucans possess immune-modulating activities? Mol. Nutr. Food Res. 2012;56:536–547. doi: 10.1002/mnfr.201100723. - DOI - PubMed
    1. Jaehrig S.C., Rohn S., Kroh L.W., Fleischer L.G., Kurz T. In vitro protential antioxidant activity of (1→3), (1→6)-β-d-glucan and protein fractions from Saccharomyces cerevisiae cell walls. J. Agric. Food Chem. 2007;55:4710–4716. doi: 10.1021/jf063209q. - DOI - PubMed
    1. Medeiros S.D.V., Cordeiro S.L., Cavalcanti J.E.C., Melchuna K.M., Lima A.M.S., Filho I.A. Effects of purified Saccharomyces cerevisiae (1→3)-β-glucan on venous ulcer healing. Int. J. Mol. Sci. 2012;13:8142–8158. doi: 10.3390/ijms13078142. - DOI - PMC - PubMed
    1. Hofer M., Pospíšil M. Modulation of animal and human hematopoiesis by β-glucans: A review. Molecules. 2011;16:7969–7979. doi: 10.3390/molecules16097969. - DOI - PMC - PubMed

Publication types

MeSH terms

Feedback