NOCA-1 functions with γ-tubulin and in parallel to Patronin to assemble non-centrosomal microtubule arrays in C. elegans

Elife. 2015 Sep 15;4:e08649. doi: 10.7554/eLife.08649.

Abstract

Non-centrosomal microtubule arrays assemble in differentiated tissues to perform mechanical and transport-based functions. In this study, we identify Caenorhabditis elegans NOCA-1 as a protein with homology to vertebrate ninein. NOCA-1 contributes to the assembly of non-centrosomal microtubule arrays in multiple tissues. In the larval epidermis, NOCA-1 functions redundantly with the minus end protection factor Patronin/PTRN-1 to assemble a circumferential microtubule array essential for worm growth and morphogenesis. Controlled degradation of a γ-tubulin complex subunit in this tissue revealed that γ-tubulin acts with NOCA-1 in parallel to Patronin/PTRN-1. In the germline, NOCA-1 and γ-tubulin co-localize at the cell surface, and inhibiting either leads to a microtubule assembly defect. γ-tubulin targets independently of NOCA-1, but NOCA-1 targeting requires γ-tubulin when a non-essential putatively palmitoylated cysteine is mutated. These results show that NOCA-1 acts with γ-tubulin to assemble non-centrosomal arrays in multiple tissues and highlight functional overlap between the ninein and Patronin protein families.

Keywords: C. elegans; NOCA-1; PTRN-1; Patronin; cell biology; developmental biology; ninein; non-centrosomal microtubule array; stem cells.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Caenorhabditis elegans / physiology*
  • Caenorhabditis elegans Proteins / metabolism*
  • Cell Cycle Proteins / metabolism*
  • Cytoskeletal Proteins / metabolism*
  • Microtubule-Associated Proteins / metabolism*
  • Microtubules / metabolism*
  • Nuclear Proteins / metabolism*
  • Protein Multimerization*
  • Tubulin / metabolism*

Substances

  • Caenorhabditis elegans Proteins
  • Cell Cycle Proteins
  • Cytoskeletal Proteins
  • Microtubule-Associated Proteins
  • NOCA-1 protein, C elegans
  • Nuclear Proteins
  • PTRN-1 protein, C elegans
  • Tubulin