1H, 15N and 13C backbone assignments of GDP-bound human H-Ras mutant G12V

Biomol NMR Assign. 2016 Apr;10(1):121-3. doi: 10.1007/s12104-015-9649-4. Epub 2015 Sep 15.

Abstract

Harvey Ras (H-Ras) is a membrane-associated GTPase with critical functions in cell proliferation and differentiation. The G12V mutant of H-Ras is one of the most commonly encountered oncoproteins in human cancer. This mutation disrupts the GTPase activity of H-Ras, leading to constitutive activation and aberrant downstream signalling. Here we report the backbone resonance assignments of human H-Ras mutant G12V lacking the C-terminal membrane attachment domain.

Keywords: Cancer; H-Ras; H-Ras G12V; NMR; NMR resonance assignments; Ras.

MeSH terms

  • Guanosine Diphosphate / metabolism*
  • Humans
  • Mutant Proteins / chemistry*
  • Mutant Proteins / genetics
  • Mutant Proteins / metabolism*
  • Mutation*
  • Nuclear Magnetic Resonance, Biomolecular*
  • Protein Binding
  • Proto-Oncogene Proteins p21(ras) / chemistry*
  • Proto-Oncogene Proteins p21(ras) / genetics
  • Proto-Oncogene Proteins p21(ras) / metabolism*

Substances

  • Mutant Proteins
  • Guanosine Diphosphate
  • Proto-Oncogene Proteins p21(ras)