CDK1 phosphorylation of TAZ in mitosis inhibits its oncogenic activity

Oncotarget. 2015 Oct 13;6(31):31399-412. doi: 10.18632/oncotarget.5189.

Abstract

The transcriptional co-activator with PDZ-binding motif (TAZ) is a downstream effector of the Hippo tumor suppressor pathway, which plays important roles in cancer and stem cell biology. Hippo signaling inactivates TAZ through phosphorylation (mainly at S89). In the current study, we define a new layer of regulation of TAZ activity that is critical for its oncogenic function. We found that TAZ is phosphorylated in vitro and in vivo by the mitotic kinase CDK1 at S90, S105, T326, and T346 during the G2/M phase of the cell cycle. Interestingly, mitotic phosphorylation inactivates TAZ oncogenic activity, as the non-phosphorylatable mutant (TAZ-S89A/S90A/S105A/T326A/T346A, TAZ-5A) possesses higher activity in epithelial-mesenchymal transition, anchorage-independent growth, cell migration, and invasion when compared to the TAZ-S89A mutant. Accordingly, TAZ-5A has higher transcriptional activity compared to the TAZ-S89A mutant. Finally, we show that TAZ-S89A or TAZ-5A (to a greater extent) was sufficient to induce spindle and centrosome defects, and chromosome misalignment/missegregation in immortalized epithelial cells. Together, our results reveal a previously unrecognized connection between TAZ oncogenicity and mitotic phospho-regulation.

Keywords: CDK1; Hippo pathway; TAZ; mitotic defects; mitotic phosphorylation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Blotting, Western
  • CDC2 Protein Kinase
  • Cell Movement
  • Cell Transformation, Neoplastic / pathology*
  • Cells, Cultured
  • Cyclin-Dependent Kinases / metabolism*
  • Epithelial-Mesenchymal Transition
  • Fluorescent Antibody Technique
  • HEK293 Cells
  • Humans
  • Immunoenzyme Techniques
  • Immunoprecipitation
  • Mitosis / physiology*
  • Neoplasms / pathology
  • Neoplasms / prevention & control*
  • Phosphorylation
  • Signal Transduction
  • Transcription Factors / metabolism*

Substances

  • Transcription Factors
  • TAFAZZIN protein, human
  • CDC2 Protein Kinase
  • CDK1 protein, human
  • Cyclin-Dependent Kinases