De novo production of six key grape aroma monoterpenes by a geraniol synthase-engineered S. cerevisiae wine strain

Microb Cell Fact. 2015 Sep 16;14:136. doi: 10.1186/s12934-015-0306-5.


Background: Monoterpenes are important contributors to grape and wine aroma. Moreover, certain monoterpenes have been shown to display health benefits with antimicrobial, anti-inflammatory, anticancer or hypotensive properties amongst others. The aim of this study was to construct self-aromatizing wine yeasts to overproduce de novo these plant metabolites in wines.

Results: Expression of the Ocimum basilicum (sweet basil) geraniol synthase (GES) gene in a Saccharomyces cerevisiae wine strain substantially changed the terpene profile of wine produced from a non-aromatic grape variety. Under microvinification conditions, and without compromising other fermentative traits, the recombinant yeast excreted geraniol de novo at an amount (~750 μg/L) well exceeding (>10-fold) its threshold for olfactory perception and also exceeding the quantities present in wines obtained from highly aromatic Muscat grapes. Interestingly, geraniol was further metabolized by yeast enzymes to additional monoterpenes and esters: citronellol, linalool, nerol, citronellyl acetate and geranyl acetate, resulting in a total monoterpene concentration (~1,558 μg/L) 230-fold greater than that of the control. We also found that monoterpene profiles of wines derived from mixed fermentations were found to be determined by the composition of the initial yeast inocula suggesting the feasibility of producing 'à la carte' wines having predetermined monoterpene contents.

Conclusions: Geraniol synthase-engineered yeasts demonstrate potential in the development of monoterpene enhanced wines.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fermentation
  • Metabolic Engineering*
  • Monoterpenes / metabolism*
  • Ocimum basilicum / enzymology
  • Odorants*
  • Phosphoric Monoester Hydrolases / genetics
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*
  • Vitis / chemistry*
  • Wine*


  • Monoterpenes
  • Plant Proteins
  • Recombinant Proteins
  • Phosphoric Monoester Hydrolases