Inhibition of DNA nanotube-conjugated mTOR siRNA on the growth of pulmonary arterial smooth muscle cells

Zaichun You; Hang Qian; Changzheng Wang; Binfeng He; Jiawei Yan; Chengde Mao; Guansong Wang

doi:10.1016/j.dib.2015.08.002

Inhibition of DNA nanotube-conjugated mTOR siRNA on the growth of pulmonary arterial smooth muscle cells

Data Brief. 2015 Aug 15:5:28-34. doi: 10.1016/j.dib.2015.08.002. eCollection 2015 Dec.

Authors

Zaichun You¹, Hang Qian², Changzheng Wang³, Binfeng He³, Jiawei Yan², Chengde Mao⁴, Guansong Wang³

Affiliations

¹ Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China ; Department of Emergency, Xinqiao Hospital, Chongqing 400037, China.
² State Key Lab of Physical Chemistry of Solid Surfaces, and Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
³ Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
⁴ Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.

Abstract

Here we provide raw and processed data and methods behind mTOR siRNA loaded DNA nanotubes (siRNA-DNA-NTs) in the growth of pulmonary arterial smooth muscle cells (PASMCs) under both normoxic and hypoxic condition, and also related to (You et al., Biomaterials, 2015, 67:137-150, [1]). The MTT analysis, Semi-quantitative RT-PCR data presented here were used to probe cytotoxicity of mTOR siRNA-DNA-NT complex in its TAE-Mg(2+) buffer. siRNA-DNA-NTs have a lower cytotoxicity and higher transfection efficiency and can, based on inhibition of mTOR expression, decrease PASMCs growth both hypoxic and normal condition.

Keywords: Anoxia; Cytotoxicity; DNA nanotubes; Growth; Pulmonary arterial smooth muscle cells; mTOR siRNA.