Low level laser therapy reduces acute lung inflammation without impairing lung function

J Biophotonics. 2016 Dec;9(11-12):1199-1207. doi: 10.1002/jbio.201500113. Epub 2015 Sep 18.


Acute lung injury is a condition characterized by exacerbate inflammatory reaction in distal airways and lung dysfunction. Here we investigate the treatment of acute lung injury (ALI) by low level laser therapy (LLLT), an effective therapy used for the treatment of patients with inflammatory disorders or traumatic injuries, due to its ability to reduce inflammation and promote tissue regeneration. However, studies in internal viscera remains unclear. C57BL/6 mice were treated with intratracheal lipopolysaccharide (LPS) (5 mg/kg) or phosphate buffer saline (PBS). Six hours after instillation, two groups were irradiated with laser at 660 nm and radiant exposure of 10 J/cm2 . Intratracheal LPS inoculation induced a marked increase in the number of inflammatory cells in perivascular and alveolar spaces. There was also an increase in the expression and secretion of cytokines (TNF-α, IL-1β, IL-6,) and chemokine (MCP-1). The LLLT application induced a significant decrease in both inflammatory cells influx and inflammatory mediators secretion. These effects did not affect lung mechanical properties, since no change was observed in tissue resistance or elastance. In conclusion LLLT is able to reduce inflammatory reaction in lungs exposed to LPS without affecting the pulmonary function and recovery.

Keywords: acute lung injury; low level laser therapy; lung function; photobiomodulation.

MeSH terms

  • Acute Lung Injury / radiotherapy*
  • Animals
  • Chemokines / metabolism
  • Cytokines / metabolism
  • Inflammation / radiotherapy*
  • Low-Level Light Therapy*
  • Lung / physiopathology
  • Lung / radiation effects
  • Mice
  • Mice, Inbred C57BL


  • Chemokines
  • Cytokines