Dam it's good! DamID profiling of protein-DNA interactions

Wiley Interdiscip Rev Dev Biol. Jan-Feb 2016;5(1):25-37. doi: 10.1002/wdev.205. Epub 2015 Sep 18.

Abstract

The interaction of proteins with chromatin is fundamental for several essential cellular processes. During the development of an organism, genes must to be tightly regulated both temporally and spatially. This is achieved through the action of chromatin-binding proteins such as transcription factors, histone modifiers, nucleosome remodelers, and lamins. Furthermore, protein-DNA interactions are important in the adult, where their perturbation can lead to disruption of homeostasis, metabolic dysregulation, and diseases such as cancer. Understanding the nature of these interactions is of paramount importance in almost all areas of molecular biological research. In recent years, DNA adenine methyltransferase identification (DamID) has emerged as one of the most comprehensive and versatile methods available for profiling protein-DNA interactions on a genomic scale. DamID has been used to map a variety of chromatin-binding proteins in several model organisms and has the potential for continued adaptation and application in the field of genomic biology. WIREs Dev Biol 2016, 5:25-37. doi: 10.1002/wdev.205 For further resources related to this article, please visit the WIREs website.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Chromatin / physiology
  • Chromatin / ultrastructure
  • Chromatin Immunoprecipitation
  • Chromosomal Proteins, Non-Histone / physiology*
  • Epigenesis, Genetic
  • Humans
  • Molecular Probe Techniques*
  • Molecular Sequence Annotation
  • Protein Binding
  • Site-Specific DNA-Methyltransferase (Adenine-Specific) / physiology

Substances

  • Chromatin
  • Chromosomal Proteins, Non-Histone
  • Site-Specific DNA-Methyltransferase (Adenine-Specific)